PPT-DNA Replication

C483 Spring 2013 1 Double stranded DNA that has been labeled with radioactive 14C is used as the template for replication Replication is carried out in a medium containing only unlabeled nucleotides After two rounds of replication what percent of . WHAT YOU WILL LEARN. -process of DNA making copies . -role of enzymes in copying DNA. -leading and lagging strands in DNA replication. READING Q. ’. s . 1-LIST:. -the 3 phases of semi-conservative replication. 20.1 part 2. DNA replication. Earlier on in this unit we learned about mitosis or cell division.. In order for mitosis to occur and chromosomes to duplicate themselves, the DNA must copy itself exactly.. Chapter . 7.2. Processing of Genetic Material. What is DNA Replication. The process by which the DNA within a cell makes exact copies of itself. Balance of speed and accuracy. Used for growth, repair and embryonic development. Learning Objectives. What happens during DNA replication?. How is a new polynucleotide strand formed?. Why is DNA replication known as ‘semi-conservative’?. Putting What We’ve Learnt So Far In To Context.... Objectives:. 1. Summarize the roles of the different enzymes involved in replication of DNA.. 2. Explain how the leading and lagging strands are replicated differently . Semiconservative. Replication. DNA REPLICATION. DNA IS COPIED. 1. DURING . S-phase. of . INTERPHASE. PREPARATION FOR CELL DIVISION. SEPARATION. 2. THE . STrAND. OF DNA IS . UNZIPPED. BY . HELICASE. 3. THE POINT OF SEPARATION IS CALLED THE . . §. 4.3a DNA Replisome. . §. 4.3b DNA Damage. §. 4.3c DNA . Repair. IV. NUCLEAR BIOCHEMISTRY. §. 4.3a . DNA Replisome. Synopsis . 4.3a. DNA replication involves making . copies or “. replicas” of DNA. Yadi. Zhou. 2013.12.05. RecA. protein. 38 . kiloDalton. Essential for the repair and maintenance of DNA. A . RecA. structural and functional homolog has been found in every . species. Homologous protein in . Need to Book. Speakers. A4 paper (coloured). Pair of Compass & Pencils. Split pins. Scissors. Repro. Meselson. and . Stalh. hand out. Describe semi-conservative DNA replication. Learning Objective. By. Dr. R. C. . Nath. ; Associate Professor. Department of Zoology. Govt. Degree College, DMR. .. Structure of a DNA molecule. `. It . has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material. 9.1 DNA Replication is Semiconservative. DNA Replication is Semiconservative. DNA Replication is Semiconservative. Chapter 9: DNA Replication . 9.2 DNA Replication in Prokaryotes. Prokaryotic DNA is circular. Study Material for. . . B.Sc. Part II. . Botany . Hons. .. . . Paper IV. . Dr. Pushpanjali Khare. Sr. . Directions: Front of card write term. On back write definition and draw picture . Chromosomes. Thread-like structure made of coiled DNA. Gene. Segment of DNA that codes for a specific trait. Nucleotide. . REPLICATION – GENERAL PRINCIPLES. START. Must be ready . Must know where to start . FINISH. Must all finish . Must ensure that each piece of DNA is replicated only once . Therefore must know where to finish .