EG1003 LAB 11 OBJECTIVE Use basic forensic techniques DNA extraction Fingerprinting Drug testing Blood typing Solve a murder mystery 2 Figure 2 Fingerprinting Courtesy of Marian University ID: 1045559
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1. BIOMEDICAL FORENSICS(VIRTUAL)EG1003 | LAB 11
2. OBJECTIVEUse basic forensic techniquesDNA extractionFingerprintingDrug testingBlood typingSolve a murder mystery2Figure 2: Fingerprinting Courtesy of Marian University
3. BACKGROUND INFORMATIONDeoxyribonucleic acid (DNA)Packaged in the form of chromatin (which make up chromosomes)Exist in the nuclei of cellsPlant cells have a cell wall that must be penetrated to access the DNA3Figure 3: DNA Packaging Sequence Courtesy of Byju’s
4. BACKGROUND INFORMATIONDNA extraction (of a plant cell)Break the cell wall (cell lysis) to expose the DNA within the cellDestroy the plasma membrane to further expose the DNAPrecipitate the DNA to make the DNA visible4Figure 4: Plant Cell Structure Courtesy of Biology Wise
5. BACKGROUND INFORMATIONFingerprints – unique patterns consisting of friction ridges and burrowsLoops – 60% of pattern typesWhorls – 35% of pattern types Arches – 5% of pattern typesFigure 5: Fingerprint Types Courtesy of Headlines on Human Hands5
6. BACKGROUND INFORMATIONBlood typing (ABO system)Existence of antigens on red blood cells determine blood typeA and B antigens determine type of blood (A, B, AB, or O)Rh antigen determines presence of Rhesus factor (indicated by +/-)6Figure 6: ABO Blood Type System Courtesy of Buzzle.com
7. MATERIALSComputer with internet accessPaperPencil with graphiteClear packaging tape7
8. PROCEDUREFollow the videos and procedure to learn biomedical forensic techniquesForensic Academy:DNA extractionFingerprintingDrug testing8
9. PROCEDURESolve a murder mysteryCrime Scene Investigation:Drug testingBlood typingFingerprintingCompare data to the Suspect Information SheetDetermine who is the killer9
10. ASSIGNMENTTeam lab report:Answer discussion questions in manualInclude screenshots of extracted DNA, fingerprint on murder weapon, blood typing of suspects, and drug present in victim’s bloodInclude the fingerprint and blood type of the killerDue at 11:59 PM the night before next lab sessionTeam presentation:Address discussion points in manualRely heavily on graphics and pictures10
11. CLOSINGTake screenshots of the extracted DNA, fingerprints, blood typing, and drug present in victim’s bloodRecord data in the Suspect Information SheetTell a TA who you think the killer is before leaving labSubmit all work electronically11
12. QUESTIONS?