PPT-Gel Electrophoresis BIOTECHNOLOGY

One of the basic tools of modern biotechnology is gene splicing This is the process of removing a functional DNA fragment a gene from one organism and combining it with the DNA of another organism to study how the gene works. Ayat. . Zawawi. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . What is it, and . how does it work?. Technique used to separate samples of DNA, RNA, and protein according to charge and/or size. Smaller molecules move farther and faster through the . agarose. gel. DNA & Biotechnology. Biotechnology. Recombinant DNA. Gene . splicing. Restriction enzymes. Sticky ends. L. igase. & DNA-. ase. DNA sequencing. Gene probes. DNA profiling. Short tandem repeats. Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. how does it work?. Technique used to separate samples of DNA, RNA, and protein according to charge and/or size. Smaller molecules move farther and faster through the . agarose. gel. Opposite charges each other. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Today’s Experiment. Restriction Enzymes allow researchers precisely cut DNA. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Proteins are everywhere!. Proteins are diverse molecules that perform important functions within a cell. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. Biotechnology. Definition: . The use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life.. Genetic engineering . makes it possible to transfer DNA sequences from one organism to another.