PPT-Gel Electrophoresis: Introduction and Techniques

Martin Cole isoelectric focusing Mcolisi Dlamini Faraz Khan April 18 2012 Physics 200 Molecular Biophysics httpvadlocomcartoonsphpid445 What does it do Separation of. By Andrew Gioe and Ben Berger. Electrophoretic Experiments. Free Electrophoresis or Moving Boundary Electrophoresis. Done in solution with no support Medium. No longer widely used due to problems resulting from the formation of convection currents in the solution from heating.. 1. Dr. Nikhat Siddiqi. A molecule with a net charge will move in an electric field.. This phenomenon, termed . electrophoresis,. offers a powerful means of separating proteins and other macromolecules, such as . Tutorial. lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. October 15. th. – October 19. th. , 2012. Gel Electrophoresis. The process by which electricity is used to separate charged molecules (. DNA fragments, RNA, and proteins. ) based on there size, shape, and charge. . Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. School . of . Biological . Sciences. College of . Science, University of Canterbury. commons.wikimedia.org/wiki/. File:Eukaryote_DNA.svg. DNA = instructions for life. Electrophoresis separates DNA fragments according to their size. . . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . BCH 333 [practical]. Lab# 8. Objective:. -To be familiar with Agarose gel electrophoresis principle. . Agarose gel electrophoresis:. is a method of gel electrophoresis used in biochemistry and molecular biology to separate and analyze DNA or RNA molecules by size.. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1 2 Gel Electrophoresis, Principle, Types and Applications Description of Module Subject Name Paper Name Module Name/Title Gel Electrophoresis, Principle, Types and Applications Dr. Vijaya Khader Dr. By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms. Biotechnology. Definition: . The use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life.. Genetic engineering . makes it possible to transfer DNA sequences from one organism to another. Tassios PT, Chadjichristodoulou C, Lambiri M, Kansouzidou-Kanakoudi A, Sarandopoulou Z, Kourea-Kremastinou J, et al. Molecular Typing of Multidrug-Resistant Salmonella Blockley Outbreak Isolates from Greece. Emerg Infect Dis. 2000;6(1):60-64. https://doi.org/10.3201/eid0601.000111.