PPT-Polyacrylamide Gel Electrophoresis

By Angel Luis Vázquez Maldonado November 8 th 2018 Gel Electrophoresis and its Purpose 2 Electrophoresis is derived from Greek Electro refers to the electrical current that adds energy to the electrons of the molecules atoms. By Andrew Gioe and Ben Berger. Electrophoretic Experiments. Free Electrophoresis or Moving Boundary Electrophoresis. Done in solution with no support Medium. No longer widely used due to problems resulting from the formation of convection currents in the solution from heating.. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. Using Gelatin Solutions. Gregory J. Samuel, Jr.. gja9@wildcats.unh.edu ; . Parsons Hall, 23 Academic Way, Durham, NH 03824. Introduction/Background. Encapsulation processes are beneficial in a variety of fields from pharmaceuticals to food preparation. The objective of this study was to develop a shell to encapsulate a 10% wt. polyacrylamide gel. The shell needed to be impermeable enough to retain most of the water content, while still maintaining the ability to burst under pressure and release the contents over a surface. Preliminary testing (Method 1) was performed using commercially available 0.50 caliber (0.50 inch diameter) gelatin-shell paintballs. These capsules seemed to demonstrate the desired capsule properties, prompting further studies of gelatin encapsulation. Three solutions containing gelatin, water, and glycerol were prepared and tested (Method 2) to determine an optimal gelatin recipe for encapsulation of the polyacrylamide gel.. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. IUG, . Spring . 2015. TMZ. 1. SDS-PAGE . Part I. 2. SDS-PAGE . (. PolyAcrylamide. Gel Electrophoresis). SDS-PAGE. ,. . sodium . dodecyl. sulfate . polyacrylamide. gel electrophoresis. , is a technique widely used in biochemistry, forensics, genetics and molecular biology:. School . of . Biological . Sciences. College of . Science, University of Canterbury. commons.wikimedia.org/wiki/. File:Eukaryote_DNA.svg. DNA = instructions for life. Electrophoresis separates DNA fragments according to their size. . Part IV). By:. Dr.. R. K. . Jaiswal. Asstt. . Prof.-cum-Jr. Scientist. Dept. of LPT. Bihar Veterinary College. Bihar Animal Sciences University. Patna-800014 (Bihar). LPT . 602: . MEAT. . PROCESSING. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac GE. By. MSc. . Elham. . Faisal. MSc.Wafaa. . Ajam. Analytical chemistry(practical) . Ministry of higher education and scientific research. AL-. Mustaqbal. University college . Department of medical physics. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . . TOPIC: GEL ELECTROPHORESIS. Gel Electrophoresis. Gel electrophoresis is a method that separates macromolecules, either nucleic acid or protein.. Electrophoresis describes the migration of charged particles under the influence of an electric field.. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:.