PPT-Protein separation by SDS – PAGE

Presented by DrKANBARASAN Assistant professor and Head Department of Botany Padmavani Arts and Science College for Women Salem 636 011 ELECTROPHORESIS Electrophoresis is a molecular technique widely used in field of biochemistry molecular biology and biotechnology to separate the bio molecules based on the migration of charged molecules. Tutorial. lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. Poor Protein Solubility carry no net charge. The ionic strength of the mediumis also very low, because small ions have all migrated High Protein Load When a large quantity of protein is loaded, or whe dodecyl. sulfate – . polyacrylamide. gel electrophoresis . (SDS-PAGE). Provides a means to look at all the proteins in a cell simultaneously. Electrophoretic mobility of a protein depends on its molecular weight. . 생명과학부. 구용의. M. ass . S. pectrometer(MS). 분자가 . MS . 내로 들어가면 분자는 이온화됨과 동시에 더 작은 이온들. (fragments). 로 쪼개진다. . . 쪼개진 이온들은 그들의 질량. Lecture . 7. 1. Protein - Characterization. 2. Absorbance Spectroscopy. :. The aromatic amino acids all have characteristic absorbance profiles. 5500 M. -1. cm. -1. 1490 M. -1. cm. -1. Also Cysteine. S. pectrometer(MS). 분자가 . MS . 내로 들어가면 분자는 이온화됨과 동시에 더 작은 이온들. (fragments). 로 쪼개진다. . . 쪼개진 이온들은 그들의 질량. /. 전. 하. Drug Substance. Separation and Purification. Wisit Tangkeangsirisin, PhD. Faculty of Pharmacy. Silpakorn University. Cell Disruption. Cell separation. Centrifugation. Chromatography . ion. -. exchange. Curated Protein Information in the Saccharomyces Genome Database Sage Hellerstedt 3/27/17 Post Translational Modification Data (PTM) Curation Strategy 1. Identify articles with PTM data 2. Data Extraction WITH AN OPPOSITE HANDEDNESS . BY OLIGOPEPTIDE ADSORPTION:. A MOLECULAR DYNAMICS STUDY. Giuseppina Raffaini. Dipartimento di Chimica, Materiali e Ing. Chimica “G. Natta”. Politecnico di Milano - Italy. . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. Marker can be used to determine size of sample. 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . BCH . 462 [practical. ] . 4. th. Lab. Objectives:. -Separation of protein fractions using SDS-PAGE.. -Sodium . Dodecyl . Sulfate. -Polyacrylamide . gel Electrophoresis (SDS-PAGE. ), . is a technique widely used in biochemistry ,forensics, genetics and molecular biology to separate and identify proteins . Dr. D Y Patil Pimpri Pune 411018 Chemistry Department Mrs. Tarannum S Attar CONTENT Introduction Principle Factors affecting Conventional electrophoresis General operation Technical and prac