PPT-ELECTROPHORESIS INTRODUCTION

Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field The usual purposes of carrying out electrophoretic experiments are 1 To determine the number amount and mobility of components in a given sample or to separate them. 1. Dr. Nikhat Siddiqi. A molecule with a net charge will move in an electric field.. This phenomenon, termed . electrophoresis,. offers a powerful means of separating proteins and other macromolecules, such as . EDVOTEK Tutorial. lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. Native PAGEIEF AgaroseyesyesSDS-PAGEBlotting after electrophoresis PAGE gelsyesyes . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1 Electrophoresis is a general term that describe the migration and separation of charge particles under the influence of an electric field. The particle may be simple ions, complex macromolecules 4 LA842 (2 Terminal) LA690 (4 Terminal) Accessories Connecting Cable: 1 Set (Black & Red) Output Range : 10 to 300 VDC Accessories Connecting Cable: 1 Set (Black & Red) Output Range : 50 / 100 VDC P To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac Dr. NISHA SHARMA, ASSOCIATE PROFESSOR, C.S.J.M. UNIVERSITY, KANPUR. 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. PAPER ELECTROPHORESIS. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Immunofixation. Dr.Ajay. . Phadke. Centre Head. SRL Diagnostics-. Dr.Avinash. . Phadke’s. Lab. What is electrophoresis?. Electrophoresis is a method of separating proteins based on their physical properties. By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:.