PPT-Agarose gel electrophoresis

BCH 333 practical Lab 8 Objective To be familiar with Agarose gel electrophoresis principle Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry and molecular biology to separate and analyze DNA or RNA molecules by size. and Analysis. Activity 4.3: Dye Electrophoresis . Activity 4.3: Dye Electrophoresis. Research questions:. What charge do dyes have?. Are samples composed of multiple dyes?. Objectives:. Load and run dyes on an agarose gel. Bio-Rad Biotechnology . Explorer™ . DNA Fingerprinting Kit. Crime Scene. Have fun setting up your own crime scene. Be as elaborate or as simple as you wish.. Dye Electrophoresis . Could you eliminate any suspects using dye electrophoresis?. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . One of the basic tools of modern biotechnology is gene splicing.. This is the process of removing a functional DNA fragment ( a gene) from one organism and combining it with the DNA of another organism to study how the gene works.. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . Objective: To visualize pieces of DNA by size, using a gel matrix and an electrical current. Background The chromosomes in our cell nuclei consist of large strands of DNA. These strands are very u Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. 1. The spectrophotometer cannot tell you if your DNA or RNA are intact, undamaged. Vigorous shaking, pipetting up and down, and . vortexing. , can damage DNA, breaking it into smaller pieces. To be sure it is not damaged, you can run a small amount, 100-200 ng, on a gel . . TOPIC: GEL ELECTROPHORESIS. Gel Electrophoresis. Gel electrophoresis is a method that separates macromolecules, either nucleic acid or protein.. Electrophoresis describes the migration of charged particles under the influence of an electric field.. TOPIC: ELECTROPHORESIS. HANDLED BY: RM.LAKSHMANAN. ASSOCIATE PROFESSOR & HEAD. DEPARTMENT OF MICROBIOLOGY. HAJEE KARUTHA . ROWTHER HOWDIA . COLLEGE(AUTONOMOUS), . UTHAMAPALAYAM . ELECTROPHORESIS.