PPT-Difference between Gram-Positive and Gram-Negative Bacteria

Gram Positive Gram Negative GramPositive bacteria GramNegative bacteria Cell Wall A singlelayered smooth cell wall  A doublelayered wavy cellwall Cell Wall thickness The thickness of the cell wall is 20 to 80 . Part-2. Sulfur Dioxide(SO2) and Sulfites(SO3). Sulfur dioxide, Sodium sulfite(Na2SO3), Sodium bisulfide(NaHSO3), and Sodium . metabisulfite. (Na2S2O5) are used to control microorganisms and insects in soft fruits, lemon juices, beverages, sausages, pickles, and fresh shrimp.. Gram positive cocci. Bile-Esculin. Enterococcus. identification. Esculin. .  . Esculitin. + Glucose. esculitin. + Fe  . Ferric. citrate (. dark. . brown. ). Selective. media. Bile . added. Bacteria . One of our kingdoms. Bacteria. Bacteria are minute microscopic prokaryotes. They are unicellular organisms, which are usually identified by their specific activities, more than their general . Lecturer : Dr. . Thanaa. . Rasheed. Historical Introduction Concerning the Development of the Science of Microbiology.. -. The golden age of Microbiology began after the year 1850. . Many scientists contribute to the science of Microbiology.. Physical differences & clinical . signifigance. . . Figure 4.8b. Gram- positive bacteria. Gram-negative bacteria. Figure 4.8a. Gram-Positive cell walls. Teichoic acids:. Lipoteichoic acid links to plasma membrane. continued. Gram Stain. When bacteria are treated with a dye made up of crystal violet and iodine they react in one of two ways. . Bacteria . cells that appear purple are gram-positive and bacteria that change to pink are gram-negative. .  . Bacteria form a large group of parasitic, saprophytic, and free-living microorganism .  . 1. Bacterial cell wall (protects against osmotic damage).  . Bacterial cell is prokaryotic. Contains high concentration . Winter 2012. Ubiquity. What organisms grow best at room temperature? ___°C? . At body temperature? = ___°C?. What kind of medium is TSA? . Appearance of growth on plates. Bacteria . vs. fungi (molds)?. Internal Structures:. cytoplasm. nucleoid. ribosomes. Boundaries:. cell membrane. cell wall. capsule. Appendages:. flagellum. pili. Shapes of Bacteria. Bacteria Identification Criteria. Shapes. Bacteriology: . study . of . bacteria. Mycology: . study . of . fungi. Protozoology: . study . of . protozoa. Phycology (or algology): . study . of . algae. Parasitology: . study . of . parasites. Immunology: . Dairy Foods Science Notes (D raft 06 - 10) The Microscopic Examination of Milk Characterization of Milk Bacteria and Cells The microscope has been used to observe and count bacteria and somatic cells 1 The slide is washed in water; excess water is removed using a blotting paper, dried in air and heat fixed before observing under microscope. Those bacteria that hold on to primary dye-iodine comple Size of a Bacterial Cell: . There are great variations in size of bacteria. . They . measure from 0.75 µ to 1.5 µ but on an average each cell of bacterium measures about 1.25 µ to 2 µ . ( . in . diameter). . Lab. . 3. By. Assistant lecturer. Zainab farooq . shafeeq. Principle. The Gram stain is the most useful and widely employed differential stain in bacteriology.. It divides bacteria in to two groups (Gram positive and Gram negative bacteria..