Fr Sing has been extensively cultivated worldwide It is a saprophytic fungus that grows on watersoaked forests logs trunks and tropical tree stumps Mushroom mycelium can produce enzymes on starch fructose sucrose and ammonium chloride into small molecules adsorbing to the fungal cell ID: 920304
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Pleurotus sajor-caju (Fr.) Sing. has been extensively cultivated worldwide. It is a saprophytic fungus that grows on water-soaked forests, logs, trunks, and tropical tree stumps. Mushroom mycelium can produce enzymes on starch, fructose, sucrose, and ammonium chloride into small molecules adsorbing to the fungal cells [1]. Lemon basil straw (Ocimum citriodorum Vis., LBS) is an agricultural waste and it was commonly eliminated by burning in the open field that impacts the climate. The LBS could be employed as the carbon source for microbials [2]. The bioconversion process by using the LBS as the alternative substrate or using the LBS water extract can persuade the farmer to reduce the burning of LBS. This study aims to determine optimal LBS extracts for the mycelial growth of P. sajor-caju in solid and liquid culture media.
Bioconversion of Lemon Basil Straw (
Ocimum citriodorum
Vis.) Extracts to Mycelium of
Pleurotus
sajor-caju (Fr.) Sing. Mushroom in Different Culture Media
Pragatsawat Chanprapai 1 and Ruengwit Sawangkeaw 1, 2*1 The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Institute Building 3, Phayathai Road, Pathumwan, Bangkok 10330, Thailand2 Research Unit in Bioconversion/Bioseparation for Value-Added Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, Thailand First author: Pragatsawat.c@chula.ac.th, *Corresponding author: Rueangwit.s@chula.ac.th
Materials And Methods
Gray oyster mushrooms (Pleurotus sajor-caju (Fr.) Singer) were collected from a mushroom farm in Bangkok, Thailand. The dried lemon basil straw (LBS) were harvested from Sukhothai.
Extraction method
Introduction
Mycelia cultivation on solid media
Table 1.
Mycelial growth of
P. sajor-caju
on different solid media of LBS extracts at room temperature (32±2
º
C)
Scale-up for mycelial production (1 Liter)
Temperature : 30 °C Agitation : 115 rpmTime : 10 daysInitial pH : 6.5Shaking flasks: 100 ml x 10 flasks
Media Mycelia Biomass (g/L)
PBD (control)
3.36 LBS (4 °C) 2.84
Conclusions
In the present work, Pleurotus sajor-caju’s mycelium could be grown on the media mixing with the LBS extracts. The LBS extracted in room temperature was represented to widely support the growth of the mushroom in solid medium. On the other hand, the LBS extracts was not suitable for submerged cultivation. Therefore, the LBS extracted in room temperature could be used to promote the mycelium growth in solid medium for industrial cultivation of P. sajor-caju in the future.
AcknowledgementsThe financial supports for this project were provided by the Chulalongkorn University Second Century Fund (C2F) of Postdoctoral Scholarship.
ReferencesDulay, R.M.R.; Cabrera, E.C.; Kalaw, S.P.; Reyes, R.G., Hou, C.T. Nutritional requirements for mycelial growth of three Lentinus species from the Philippines. Biocatal. Agric. Biotechnol 2020, 23: 1-7. Saheed, O.K.; Jamal, P.; Karim, M.I.A.; Alam, Z.; Muyibi, S.A. Utilization of fruit peels as carbon source for white rot fungi biomass production under submerged state bioconversion. J. King Saud Univ. Sci 2016, 28: 143-151.
P.
sajor-caju
O.
citriodorum
Treatment
Date and mean diameter of mycelial growth (cm)
Growth Rate (mm
2
/day)
R
22345678PDA+LBS RT1.48±.03a2.20±.18a3.01±.50a3.33±.58a3.82±.64a4.43±.93a6.90±.80a12.920.8945PDA+LBS 121°C1.17±.08b1.30±.23c2.13±.46b2.67±.47b3.73±.58a4.52±.27a6.87±.27a5.050.9157PDA+LBS 4°C1.13±.08b1.50±.05bc2.23±.38b2.45±.38b2.73±.42b3.83±.40a6.15±.62a6.960.8495PDA(Control)0.97±.15c1.67±.15b1.92±.08b2.10±.10b2.50±.10b4.17±.29a6.37±.15a7.850.9351Total6.57±.57Sig.=.30Error=.16Mean of Square (MS)0.42F=1.46
* Significant difference (p<.05, DMRT)
Mycelial cultivation in liquid media
Table 2.
Mycelia biomass and exopolysaccharides of P. sajor-caju on submerged condition based on LBS extracts for 10 days at 30±2 ºC
Media Initial pHFinal pHFresh biomass (g/100 ml)*Dried biomass (g/100 ml)*PDB (control)6.505.897.98±.22a0.54±.27aLBS RT6.506.393.21±.12c0.12±.01bLBS 4°C6.505.708.30±.17a0.35±.01abLBS 121°C6.506.485.10±.17b0.30±.02abTotal6.15±2.200.33±.20F-test**%C.V.35.8360.61
* Significant difference (p<.05, DMRT)
LBS 4
º
C
PDB
Results
Mushroom isolation
M
ycelial growth on solid media
M
ycelial production
in liquid media (100 ml)
Bioconversion
Mushroom collection
Cleansing the surface by 70% ethanol
Ripping the pileus along with the end of stipe
Cutting the internal tissues into small pieces by a sterilized knife
Cultivation in darkness at temperature of 28
◦
C
and 70% relative humidity, 7
days for subculture in the study
S
ubmerging the LBS
by DI water
Room temperature, 4 °C and 121 °C
Lemon Basil Straw (LBS)