digest 1 g of lambda DNA in 1 hour at 37C in 50 L of restriction enzymes are active in a 1X or 2X concentration Buffer Please refer to Buffer 33 mM Trisacetate pH 79 at 37C 10 mM magnesium acetate ID: 873224
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1 1000 U Lot: ___ Expiry Date: _ï¯N N N
1000 U Lot: ___ Expiry Date: _ï¯N N N A G G...3' 3'...G G A N N NïN N T C C5' Concentration: 10 U/µL Supplied with: 1 mL of 10X Buffer R 1 mL of 10X Buffer Tango digest 1 µg of lambda DNA in 1 hour at 37°C in 50 µL of restriction enzymes are active in a 1X or 2X concentration Buffer. Please refer to Buffer: 33
2 mM Tris-acetate (pH 7.9 at 37°C), 10 mM
mM Tris-acetate (pH 7.9 at 37°C), 10 mM magnesium acetate, 66 mM potassium acetate, and 50% glycerol. nuclease-free water 16 µL 10X Buffer R 2 µL DNA (0.5-1 µg/µL) 1 µL XagI 0.5-2 µL PCR reaction mixture 10 µL nuclease-free water 18 µL 10X Buffer R 2 µL XagI 1-2 µL B G O R Tango 2X Tango Dcm: may overlap no effect.
3 Digestion of Agarose-embedded DNA compl
Digestion of Agarose-embedded DNA complete digestion of 1 µg of agarose-embedded lambda pBR322 pUC57 pUC18/19 pTZ19R/U M13mp18/19 9 0 1 0 0 0 0 CERTIFICATE OF ANALYSIS Overdigestion Assay The ligation and recleavage assay was replaced with LO trace nuclease and phosphatase activities with sensitivity No detectable degrada
4 tion of single-stranded or double-strand
tion of single-stranded or double-stranded labeled oligonucleotides occured during PRODUCT USE LIMITATION The product was not tested for use in diagnostics or for drug Please refer to www.thermoscientific.com/onebio © 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the ific Inc. and its subsidiari