PPT-(PC) Genetic Mapping and Marker Development for the

Powdery Mildew Resistance Gene PMR1 in Pepper Capsicum annuum Jinkwan Jo 1 Jelli Venkatesh 1 Koeun Han 1 and ByoungCheorl Kang 1 1 Department of Plant Science Plant Genomics and Breeding Institute and Vegetable Breeding Research Center College of Agriculture and Life Sciences Seoul National University Seoul 151921 Republic of . RADseq. : Restriction-site Associated DNA markers. Uses . Illumina. sequencing technology. Based on digestion with restriction enzymes. An adapter binds to the restriction site and up to 5kb fragments are sequenced around the target size.. Genome sequencing. Next Gen sequencing. Genomics = resources. genome maps. genome sequences. genome clones. DNA microarrays. whole gene collections. functional genomics!. 1980- 1998. 1977- 2003. DNA mapping. Mrs. Stewart. Medical Interventions. Central Magnet School. Standard:. Marker analysis is a technique used to determine the presence of genetic mutations associated with cancer.. Objective. :. Investigate. Breeding . Team.  . The . VitisGen. Breeding Team is responsible for maintaining the mapping populations selected to use in . the . VitisGen. project and to . supply the . genotyping and phenotyping . How many genes determine important traits?. Where these genes are located?. How do the genes interact? . What is the role of the environment in the phenotype?. Molecular breeding: Gene discovery, characterization, and selection using molecular tools. How many genes determine important traits?. Where these genes are located?. How do the genes interact? . What is the role of the environment in the phenotype?. Molecular breeding: Gene discovery, characterization, and selection using molecular tools. petercelec@gmail.com. www.imbm.sk. Linus. . Carl. . Pauling. (1901 – 1994). Nobel. . prize. 1954 – . Chemistry. 1962 – Peace. Francis. . Crick. (1916 – 2004). Nobel. . p. rize. 1962 – . Daniel Kling & Andreas . Tillmar. daniel.kling@rmv.se. andreas.tillmar@rmv.se. Agenda . 10am-12pm. «Small» . (ForenSeq, ICMP panel. ). Markers. Typing technology. Statistical concepts of relevance. ore . A. ccurate. Truong SK, McCormick RF, . Morishige. DT, Mullet JE (2014) Resolution of genetic map expansion caused by excess heterozygosity in plant recombinant inbred populations. G3 (Bethesda) 4: 1963-1969. 1 Genetic and Physical MappingThe ultimate goal of mapping is to identify the gene(s) responsible for a given phenotype or the mutation responsible for a specific variant.The initial steps in mapping 109ABSTRACT Z. J. LiuFish Molecular Genetics and Biotechnology LaboratoryDepartment of Fisheries and Allied AquacultureAuburn University, Alabama 36848, USA LIU, Z. 2001. Gene mapping, marker-assisted cation of marker-trait associations, mapping Quantitative Trait Loci (QTL), candidate gene identi cation, basis for high-resolution genetic ne-mapping, which provide powerful tools for genetic anal Crop Improvement D. Datta Sanjeev Gupta S.K. Chaturvedi N. Nadarajan Indian Institute of Pulses Research Kanpur - 208 024 ICAR Printed : September, 2011 Published by : Dr. N. Nadarajan, Director Indi Baud, A. et al. . Rat Genome Sequencing and Mapping Consortium. Presented by Alex . Gileta. Hailing from the Palmer Lab. Nature Genetics .  . 45. , . . 767–775 (2013). Genetic Variation and Complex Traits.