PPT-Figure 1 Figure 1. A) Specificity of primers for PARV4. Samples in lanes 1–5

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Fryer JF Kapoor A Minor PD Delwart E Baylis SA Novel Parvovirus and Related Variant in Human Plasma Emerg Infect Dis 2006121151154 httpsdoiorg103201eid1201050916

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Figure 1 Figure 1. A) Specificity of primers for PARV4. Samples in lanes 1–5: Transcript


Fryer JF Kapoor A Minor PD Delwart E Baylis SA Novel Parvovirus and Related Variant in Human Plasma Emerg Infect Dis 2006121151154 httpsdoiorg103201eid1201050916. Fayer R, Lewis EJ, Trout JM, Graczyk TK, Jenkins MC, Higgins J, et al. Cryptosporidium parvum in Oysters from Commercial Harvesting Sites in the Chesapeake Bay. Emerg Infect Dis. 1999;5(5):706-710. https://doi.org/10.3201/eid0505.990513. Kochar DK, Saxena V, Singh N, Kochar SK, Kumar SV, Das A. Plasmodium vivax Malaria. Emerg Infect Dis. 2005;11(1):132-134. https://doi.org/10.3201/eid1101.040519. Ndip LM, Bouyer DH, Da Rosa AP, Titanji V, Tesh RB, Walker DH. Acute Spotted Fever Rickettsiosis among Febrile Patients, Cameroon. Emerg Infect Dis. 2004;10(3):432-437. https://doi.org/10.3201/eid1003.020713. Sendow I, Field H, Curran J, Morrissy C, Meehan G, Buick T, et al. Henipavirus in Pteropus vampyrus Bats, Indonesia. Emerg Infect Dis. 2006;12(4):711-712. https://doi.org/10.3201/eid1204.051181. Torres J, Andréoletti O, Lacroux C, Prieto I, Lorenzo P, Larska M, et al. Classical Bovine Spongiform Encephalopathy by Transmission of H-Type Prion in Homologous Prion Protein Context. Emerg Infect Dis. 2011;17(9):1636-1644. https://doi.org/10.3201/eid1709.101403. Figure 6.01. Polymerase Chain Reaction (PCR). During PCR, two primers anneal to complementary sequences at either end of a target sequence on a piece of denatured sample DNA. . DNA polymerase. synthesizes a complementary strand of DNA from the primers, resulting in two new strands of DNA. In further cycles, the newly made DNA molecules are denatured, primers are annealed, and DNA polymerase copies the target regions, resulting in multiple copies of the original target sequence.. Kidd SE, Bach PJ, Hingston AO, Mak S, Chow Y, MacDougall L, et al. Cryptococcus gattii Dispersal Mechanisms, British Columbia, Canada. Emerg Infect Dis. 2007;13(1):51. https://doi.org/10.3201/eid1301.060823. Coenye T, Vandamme P, LiPuma JJ. Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction. Emerg Infect Dis. 2002;8(7):692-696. https://doi.org/10.3201/eid0807.010472. Doublet B, Lailler R, Meunier D, Brisabois A, Boyd D, Mulvey MR, et al. Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Albany. Emerg Infect Dis. 2003;9(5):585-591. https://doi.org/10.3201/eid0905.020609. Li J, Collins WE, Wirtz RA, Rathore D, Lal A, McCutchan TF. Geographic Subdivision of the Range of the Malaria Parasite, Plasmodium vivax. Emerg Infect Dis. 2001;7(1):35-42. https://doi.org/10.3201/eid0701.700035. Ley DH, Berkhoff JE, Levisohn S. Molecular Epidemiologic Investigations of Mycoplasma gallisepticum Conjunctivitis in Songbirds by Random Amplified Polymorphic DNA Analyses. Emerg Infect Dis. 1997;3(3):375-380. https://doi.org/10.3201/eid0303.970318. Cama V, Gilman RH, Vivar A, Ticona E, Ortega Y, Bern C, et al. Mixed Cryptosporidium Infections and HIV. Emerg Infect Dis. 2006;12(6):1025-1028. https://doi.org/10.3201/eid1206.060015. Kariuki S, Gilks C, Revathi G, Hart CA. Genotypic Analysis of Multidrug-Resistant Salmonella enterica Serovar Typhi, Kenya. Emerg Infect Dis. 2000;6(6):649-651. https://doi.org/10.3201/eid0606.000616. de Almeida MB, Zerbinati RM, Tateno AF, Oliveira CM, Romão RM, Rodrigues JC, et al. Rhinovirus C and Respiratory Exacerbations in Children with Cystic Fibrosis. Emerg Infect Dis. 2010;16(6):996-999. https://doi.org/10.3201/eid1606.100063.

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