PPT-Mass spectrometry-based proteomics

Jeff Johnson Feb 19 2014 MS Proteomics in a Nutshell Ionization Delivering macromolecules to the MS Ion Manipulation Trapping and ejecting analytes of interest Fragmentation Breaking apart for more information. Motivation: Mass spectrometry experiments in the field of proteomics produce lists containing tens to thousands of identified proteins. With the Protein Information and Property Explorer (PIPE) the b Keywords. :. Molecular . ion (M. +). Fragmentation. Starter:. Use mini wipe boards to brainstorm what you know about mass spectrometry. A: . Use . the molecular ion peak in an organic . molecule’s . International welcomes . submissions that are original and technically so as to serve both the developing world and developed countries in the best possible way.. OMICS Journals are poised in excellence by publishing high quality research. . Kelly Stecker. Sussman. Lab. Outline. What is MRM/SRM/Targeted mass spec. Quantification using peptide standards. Selecting standard peptides and building methods. Practical notes and suggestions. . , an improved quantification software based on . wavelet signal threshold de-noising for labeled quantitative proteomic analysis. Fan Mo. 1,*. , Qun Mo. 2,*. , Yuanyuan Chen. Courtesy www.lab-initio.com. Purpose of Mass Spectrometry. P. roduces.  . spectra of masses from . the molecules . in . a sample of . material, and fragments of the molecules.. U. sed . to determine . Orbitrap. at the Proteomics facility of the University of Stellenbosch. Salome . Smit. Central. Analytical Facility . University. of Stellenbosch. Overview. Proteomics analysis of cardiac endothelial cells. Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. Fenyő. Readings for Today. http://fenyolab.org/pi2019. Ion Source. Mass Analyzer. Detector. mass/charge. intensity. Mass spectrometry . Mass Analyzer 1. Frag-mentation. Detector. Ion Source. Mass Analyzer 2. vestigator/Program Director Last First MiddlePHS 398/2590 Rev 05/01Page Continuation Format Page1BIOGRAPHICAL SKETCHProvide the following information for the key personnel in the order listed for Form &. Secondary. . ion. . mass. . spectrometry. (SIMS). L. Seda Mut 20970802 . Neslihan . Ötük. 20622809. Beytepe. Ankara. 12.04.2012. Outline. Historical Background of MS. . and. SIMS. What is M. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. Application of proteomic. Genomics . Application of Genomics. Metabolomics. Type of metabolomics . Application of Metabolomics. Nutrigenomics. Proteomics. The study of structure and function of protein including the way they work and interact with each other inside the cells..