PPT-Outer Membrane Vesicle of Bacteria: Friend or Foe?

Presented by Seyed Davar Siadat PhD Pasteur Institute of Iran 1 WHAT ARE OMVs BASIC RESEARC 1 Small spherical structures 20250 nm in diameter 2 Produced by growing cells not products of cell . CLS 311: Basic Bacteriology. Mrs. . Amany. Ahmed . Niazy. Bacterial Structure. Bacterial Structure. Bacterial. Structure. Exterior. Structures (cell envelope). Capsule. Cell Wall. Cell Membrane. Filamentous ProteinAppendages. Extracellular Vesicle. (PMID: 23420871). Extracellular Exosome . or “exosomes” (plural) . Formed in multivesicular bodies. ~40-200 nm. (PMID. : . 6151502 ,.  . 16487731, 15261674. , . 20601276). Last day. Brief . introduction to bacteria, . Archaebacteria. , and bacterial culturing media. . Today. We will start by looking at our cultures, and providing colony descriptors. . We . will learn about the Biology of Bacteria. Bacteria, friend or foe…?. We have learned lots already about the detriments of bacteria…. They do smell bad…. Disease. Antibiotic resistance. Biofilm formation*. Spoilage. But are these microorganisms vital to our survival?.  . Bacteria form a large group of parasitic, saprophytic, and free-living microorganism .  . 1. Bacterial cell wall (protects against osmotic damage).  . Bacterial cell is prokaryotic. Contains high concentration . Figure 4.13c. Figure 4.13b–c. Gram-Positive Gram-Negative . Cell Walls Cell Walls. Thick peptidoglycan. Teichoic acids. In acid-fast cells, contains mycolic acid. Thin peptidoglycan. No teichoic acids. Bacteriology: . study . of . bacteria. Mycology: . study . of . fungi. Protozoology: . study . of . protozoa. Phycology (or algology): . study . of . algae. Parasitology: . study . of . parasites. Immunology: . Vesicular Traffic, Secretion, and Endocytosis. Chapter 14. Dr. Capers. Molecular and Cell Biology. , . Lodish. , 8. th. edition. In the previous chapter, we talked about protein transport into the mitochondria, chloroplast, peroxisome, nucleus and ER. Cell structure and Function . in Bacteria and Archaea. 1. Cell Shape and Size. 2. The . cytoplasmic. membrane and Transport. 3. Cell walls of prokaryotes. 4. Other Cellular Surface structures and inclusions. Mrs. . Amany. Ahmed . Niazy. Bacterial Structure. Bacterial Structure. Bacterial. Structure. Exterior. Structures (cell envelope). Capsule. Cell Wall. Cell Membrane. Filamentous ProteinAppendages. Size of a Bacterial Cell: . There are great variations in size of bacteria. . They . measure from 0.75 µ to 1.5 µ but on an average each cell of bacterium measures about 1.25 µ to 2 µ . ( . in . diameter). . . Bakir. Structure of bacteria . 2. It . is important to understand the basic structural properties and the physiology of micro-organisms to establish our approach to infections. . Q: . So, why is it important to know this structure and how can we benefit from that? . Borrelia burgdorferi. Space Grant Symposium Presentation. Christopher Ramirez. Debra Hansen and Petra . Fromme. 4/17/21. 1. Background. 2. What is Lyme disease?. Most common tick-borne illness in US . Gram Positive. Gram Negative. Gram-Positive bacteria. Gram-Negative bacteria. Cell Wall. A single-layered, smooth cell wall.  A double-layered, wavy cell-wall. Cell Wall thickness. The thickness of the cell wall is 20 to 80 .