Peptic digest of animal tissue - PDF document

Motility Nitrate Medium, Buffered is recommended for isolation and detection of Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) Suspend 19.5 grams in 1000 ml distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely.Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool quicklyClostridium perfringens HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60Cultural ResponseM630: Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours . Organism Inoculum Growth Motility Nitrate Cultural Response Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) Suspend 19.5 grams in 1000 ml distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely.Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool quicklyClostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) Suspend 19.5 grams in 1000 ml distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium completely.Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool quicklyClostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2**Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2**Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thedigest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 Beef extract 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringenscolonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 Disodium phosphate 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Clostridium perfringens food poisoning is one of the most common type of human foodborne illness. The foods usuallyinvolved are cooked meat or poultry products containing large number of viable cells. Clostridium perfringens is agram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause foodpoisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for thePeptic digest of animal tissue and beef extract supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base forMotility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicatesInoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Quality ControlAppearance HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 24-48 hours . Organism Inoculum(CFU) Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered HiMedia Laboratories Technical Data Cream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 24-48 hours . Organism Inoculum(CFU) Growth Motility Nitrate Clostridium absonum ATCC27555 50-100 luxuriant weakly motile weak orreaction Clostridium perfringensATCC 12924 50-100 luxuriant negative,the stabline,mediumremains clear positive, red-violet colourdevelopedminutes Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Reference 1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., before opening Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opuBacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu1.Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Md.,2.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu Revision :/ 201 HiMedia Laboratories Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu Revision :/ 201 HiMedia Laboratories Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Figui )Fe/), Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Principle And Interpretation food poisoning is one of the most common type of human foodborne illness. The foods involved are cooked meat or poultry products containing large number of viable is gram-positive, rod shaped anaerobic, spore-forming bacteria that produces enterotoxin. This toxin if ingested, can cause poisoning. Motility Nitrate Medium, Buffered formulated in accordance with FDA (1) and APHA (2), is recommended for and HM peptone B supply amino acids and other complex nitrogenous substances. Agar is added toobtain a semisolid gel that helps to demonstrate motility of the organism along the stab line of inoculation. Growth of motileorganisms extends out from the line of inoculation. The medium contains 0.5% each of glycerol and galactose to improve theconsistency of the nitrate reduction reaction with different strains of the organisms (3). Potassium nitrate serves as a base for Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in theinoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If thisis not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. NegativeIn the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu Revision :/ 201 HiMedia Laboratories Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Figui )Fe/), Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Motility Nitrate Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in inoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If is not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Motility Nitrate Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in inoculated area. After incubation, a small puffball motility may be line of inoculation. If re-incubated for compared for turbidity to an un-inoculated tube. In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu HiMfeib Mbcpsbupsift Pwu/ Mue/ Sfh/pggidf : 33, Wbeiboi Ioe/Ftu/, MCS Mbsh, Mvncbi.511186, Dvtupnfs dbsf Np/: 133.6226 pggidf : B.526,Sxbtuil Eitib Cvtioftt Pbsl,Wib Wbeiboi Ioe/ Ftu/, MCS Mbsh, Mvncbi.511186, Ioeib/ Dvtupnfs dbsf Np/: 133.6257 2929 Fnbim: Reference Revision :/ 201 HiMedia Laboratories Technical Data Utfs nvtu fotvsf tbgf eitpptbm cy bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsy pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui tbnpmf nvtu Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Figui )Fe/), Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Motility Nitrate is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu HiMfeib Mbcpsbupsift Pwu/ Mue/ Sfh/pggidf : 33, Wbeiboi Ioe/Ftu/, MCS Mbsh, Mvncbi.511186, Dvtupnfs dbsf Np/: 133.6226 pggidf : B.526,Sxbtuil Eitib Cvtioftt Pbsl,Wib Wbeiboi Ioe/ Ftu/, MCS Mbsh, Mvncbi.511186, Ioeib/ Dvtupnfs dbsf Np/: 133.6257 2929 Fnbim: Reference Revision :/ 201 HiMedia Laboratories Technical Data Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Mfuipet gps Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, F/W/, )Fet/), Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at Please refer disclaimer Overleaf. the label. formation due the product. dry ventilated Use before expiry date on the label. pg vtfe pspdfevsft io dpnft ioup Please refer disclaimer Overleaf.Motility Nitrate Medium, BufferedM630 Composition** Ingredients Gms / Litre 5.000 3.000 Galactose 5.000 Potassium nitrate 1.000 2.500 Agar 3.000 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 19.5 grams in 1000 ml purified / distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium Dispense in test tubes to make them half full. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Motility Nitrate Motility is indicated by turbidity extending out from the line of stab inoculation. Non-motile organisms grow only in inoculated area. After 3-8 hours of incubation, a small puffball of motility may be seen around the line of inoculation. If is not observed, tubes should be re-incubated for 24-48 hours and compared for turbidity to an un-inoculated tube. In the nitrate reduction test, a pink to red color develops after addition of the reagents if nitrite is present. Colour developmentindicates that nitrate reduction has occurred in the tube. Some organisms further reduce nitrite to ammonia that can be detectedby the addition of a small amount of zinc dust to the tubes exhibiting no colour. A pink colour in this part of the test indicates Inoculate 2 grams of food sample in 15 to 20 ml of Chopped Liver Broth (M606) or Tryptone Glucose Yeast Extract Broth(M952). After an incubation at 35-37°C for 20-24 hours, isolate on Perfringens Agar Base (TSC/SFP Agar Base) (M837). C.perfringens colonies are confirmed biochemically by inoculating into Motility Nitrate Medium, Buffered Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu HiMfeib Mbcpsbupsift Pwu/ Mue/ Sfh/pggidf : 33, Wbeiboi Ioe/Ftu/, MCS Mbsh, Mvncbi.511186, Dvtupnfs dbsf Np/: 133.6226 pggidf : B.526,Sxbtuil Eitib Cvtioftt Pbsl,Wib Wbeiboi Ioe/ Ftu/, MCS Mbsh, Mvncbi.511186, Ioeib/ Dvtupnfs dbsf Np/: 133.6257 2929 Fnbim: Reference Revision :/ 201 HiMedia Laboratories Technical Data Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Mfuipet gps Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo Utfs nvtu fotvsf tviubcimiuy pg uif pspevdu)t) io uifis bppmidbuipo psips up vtf/ Pspevdut dpogpsn tpmfmy up uif iogpsnbuipo dpoubiofe iouiit boe puifs sfmbufe HiMfeib™ pvcmidbuipot/ Uif iogpsnbuipo dpoubiofe io uiit pvcmidbuipo it cbtfe po pvs sftfbsdi boe efwfmppnfouxpsl boe it up uif cftu pg pvs lopxmfehf usvf boe bddvsbuf/ HiMfeib™ Mbcpsbupsift Pwu Mue sftfswft uif sihiu up nblf dibohft uptpfdigidbuipot boe iogpsnbuipo sfmbufe up uif pspevdut bu boy uinf/ Pspevdut bsf opu ioufoefe gps ivnbo ps boinbm ps uifsbpfvuid vtf cvugps mbcpsbupsy,eibhoptuid, sftfbsdi ps gvsuifs nbovgbduvsioh vtf pomy, vomftt puifsxitf tpfdigife/ Subufnfout dpoubiofe ifsfio tipvme opu HiMfeib Mbcpsbupsift Pwu/ Mue/ Sfh/pggidf : 33, Wbeiboi Ioe/Ftu/, MCS Mbsh, Mvncbi.511186, Dvtupnfs dbsf Np/: 133.6226 pggidf : B.526,Sxbtuil Eitib Cvtioftt Pbsl,Wib Wbeiboi Ioe/ Ftu/, MCS Mbsh, Mvncbi.511186, Ioeib/ Dvtupnfs dbsf Np/: 133.6257 2929 Fnbim: Reference Revision :/ 201 HiMedia Laboratories Technical Data Bacteriological Analytical Manual, Food and Drug Administration, 1995, 8th Ed., AOAC International, Gaithersburg, Bnfsidbo Pvcmid Hfbmui Bttpdibuipo, Suboebse Mfuipet gps uif Fybniobuipo pg Ebisy Pspevdut, 2978, 25ui Fe/, Jpshfotfo, J/H/, H/ M/ J/ H/, Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical DataCream to yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.3% Agar gel.Colour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2pH7.20-7.60 Cultural Response Organism Inoculum Growth Motility Nitrate Clostridium absonum ATCC 50-100 luxuriant weakly motile weak or Clostridium perfringens 50-100 luxuriant negative, positive, red- Quality ControlAppearance before opening Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at Please refer disclaimer Overleaf. before expiry the product Product performance is best if used within stated expiry period. pg vtfe pspdfevsft io dpnft ioup