Peptic digest of animal tissue - PDF document

Motility-Indole-Lysine Medium is used as an aid for the identification of members of Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) Suspend 36.52 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense into tubes in 5 HiMedia Laboratories Technical Data Please refer disclaimer Overleaf.Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseM847: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine Enterobacter aerogenes 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive Proteus mirabilis ATCC 50-100 positive, negative positive negative Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative 2. Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3. Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4. Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Technical Data Please refer disclaimer Overleaf.Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80 Cultural Response Organism Inoculum(CFU) Motility Indole Lysine Lysine Enterobacter aerogenes 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive Proteus mirabilis ATCC 50-100 positive, negative positive negative Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative Reference 1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notEwing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of ClinicalMacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams HiMedia Laboratories Technical Data Please refer disclaimer Overleaf.Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80 Cultural Response Organism Inoculum Motility Indole Lysine Lysine Enterobacter aerogenes 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive Proteus mirabilis ATCC 50-100 positive, negative positive negative Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Organism Inoculum Motility Indole Lysine Lysine Enterobacter aerogenesATCC 13048 50-100 positive,from stabline negativereaction negative positivecolour Escherichia coli ATCC25922 50-100 positive,from stabline positive, redring at theinterface ofthe mediumon addition ofKovac's reagent negative positivereaction, purplecolour Klebsiella pneumoniaeATCC 13883 50-100 negative, occasional negative positive Proteus mirabilis ATCC25933 50-100 positive,from stabline negativereaction positivebrown colourreaction at thetop negativereaction Proteus vulgaris ATCC13315 50-100 positive,from stabline positivereaction, redring at theinterface ofthe mediumon addition ofKovac's reagent positivereaction, red-brown colourreaction at thetop negative Salmonella Enteritidis ATCC 50-100 positive,from stabline negativereaction negative positivecolour Shigella flexneri ATCC12022 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Motility-Indole-Lysine Medium is used as an aid for the identification of members of Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification Enterobacteriaceae as it provides four differential reactions in a single culture tube. It is recommended to be used alongwith Triple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members ofPeptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Motility-Indole-Lysine Medium is used as an aid for the identification of members of Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 Casein enzymic hydrolysate 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 Dextrose 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of Peptic digest of animal tissue, casein enzymic hydrolysate and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyWhen inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) (3).This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella and species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. Tryptophanasedegrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentCultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne Dmsdrnaabsdrhabdad nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identificationas it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlythe stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Proteus, Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identificationit provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlythe stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase. degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to changeFor samples follow appropriate technirues for handling per established guidelines (adenrd nodmhmf HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Klebsiella pneumoniae 50-100 negative, occasional negative positive Proteus mirabilis ATCC 50-100 positive, negative positive negative Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum(CFU) Motility Indole Lysine Lysine Enterobacter aerogenesATCC 13048 50-100 positive,from stabline negativereaction negative positivecolour Escherichia coli ATCC25922 50-100 positive, positive, redthe mediumon addition ofKovac's reagent negative positive HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negativeProteus mirabilis ATCC50-100positive,from stablinenegativereactionpositivebrown colourreaction at thetopnegativereaction HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine Enterobacter aerogenes 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniaeATCC 13883 50-100 negative,the stabline occasionalreaction negative positivecolour HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative 50-100positive, HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative 50-100positive, Proteus vulgaris ATCC13315 50-100 positive,from stabline positivereaction, redring at theinterface ofthe mediumon addition ofKovac's reagent positivereaction, red-brown colourreaction at thetop negative HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams 50-100positive, Proteus vulgaris ATCC 50-100 positive, positive positive negative HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams 50-100positive, Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive,from stabline negativereaction negative positivecolour Shigella flexneri ATCC12022 50-100 negative,the stabline occasionalreaction negative negative HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum(CFU) Motility Indole Lysine Lysine Enterobacter aerogenes 50-100 positive,from stabline negativereaction negative positivecolour Escherichia coli ATCC25922 50-100 positive,from stabline positive, redring at theinterface ofthe mediumon addition ofKovac's reagent negative positivereaction, purplecolour Klebsiella pneumoniaeATCC 13883 50-100 negative,the stabline occasionalreaction negative positivecolour Proteus mirabilis ATCC2593350-100positive,growth awayfrom stablinenegativereactionpositivebrown colourreaction at thetopnegativereaction HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive,from stabline positivereaction, redring at theinterface ofthe mediumon addition ofKovac's reagent positivebrown colourreaction at thetop negativereaction Salmonella Enteritidis ATCC13076 50-100 positive,from stabline negativereaction negative positivecolour Shigella flexneri ATCC 50-100 negative,the stabline occasionalreaction negative negative HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive,growth away negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase.degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative 30 - 30sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase.degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative 30 - 30sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247.2.Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th Ed., Elsevier Science Publishing Co.,3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative 30 - 30sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase.degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing 3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative 30 - 30sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must -Hrdmadrf, G-C- Bkhmhbak Lhbrnahnknfx Ornbdctrdr Gamcannk 1 HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing 3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative - 30sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must -Hrdmadrf, G-C- Bkhmhbak Lhbrnahnknfx Ornbdctrdr Gamcannk 1 HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing 3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must -Hrdmadrf, G-C- Bkhmhbak Lhbrnahnknfx Ornbdctrdr Gamcannk 1 HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing 3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must -Hrdmadrf, G-C- Bkhmhbak Lhbrnahnknfx Ornbdctrdr Gamcannk 1HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniae 50-100 negative, occasional negative positive 50-100positive, Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (1). It is a highly useful medium in the identification as it provides four reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) This reaction can only be detected if lysine decarboxylase is not produced, which is the case with Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase.degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not1.Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing 3.Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St.4.Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must -Hrdmadrf, G-C- Bkhmhbak Lhbrnahnknfx Ornbdctrdr Gamcannk 1HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 50-100 negative, occasional negative negative sgd ornctbs Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella Enteritidis ATCC 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative Trd adenrd dwohrx casd nm sgd kaadk- User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella ATCC 13076 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella ATCC 13076 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella ATCC 13076 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 50-100 positive, positive, red negative positive Klebsiella pneumoniaeATCC 13883 50-100 negative, occasional negative positive 50-100positive, HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 25922 50-100 positive, positive, red negative positive Klebsiella pneumoniaeATCC 13883 50-100 negative, occasional negative positive 50-100positive, HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella ATCC 13076 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 25922 50-100 positive, positive, red negative positive Klebsiella pneumoniaeATCC 13883 50-100 negative, occasional negative positive 50-100positive, Please refer disclaimer Overleaf.Motility-Indole-Lysine Medium (MIL Medium)M847 Composition** Ingredients Gms / Litre 10.000 10.000 Yeast extract 3.000 L-Lysine hydrochloride 10.000 1.000 Ferric ammonium citrate 0.500 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.6±0.2**Formula adjusted, standardized to suit performance parameters otrhehdc . distilled water. Heat to boiling to dissolve the medium completely. Dispense into Principle And Interpretation MIL Medium is prepared as per the formulation of Reller and Merrett (). It is a highly useful medium in the identification as it provides four differential reactions in a single culture tube. It is recommended to be used alongTriple Sugar Iron Agar (TSI) (M021) and Urea Agar (M112) so as to enable presumptive identification of members of and yeast extract supply amino acids and other complex nitrogenoussubstances. Dextrose is a source of energy. A small amount of agar is added for demonstration of motility along the stab lineof inoculation. Growth of motile organisms extends out from the line of inoculation, while non-motile organisms grow onlyalong the stab line. Bromocresol When inoculated with an organism that ferments dextrose, acids are produced that lower the pH, causing the indicator inthe medium to change from purple to yellow. The acidic pH also stimulates decarboxylase enzyme activity. Organisms thatpossess a specific decarboxylase degrade the amino acid provided in the medium, yielding a corresponding amine. Lysinedecarboxylation yields cadaverine. The production of these amines elevates the pH and causes the medium in the bottomportion of the tube to revert to a purple color. The medium in the upper portion of the tube remains acidic because of the higheroxygen tension. If the organism being tested does not produce the required decarboxylase, the medium remains yellow (acidic)throughout or yellow with a purple or red reaction near the top. Lysine deamination produces a colour change in the upperportion of the medium. Oxidative deamination of lysine yields a compound that reacts with ferric ammonium citrate, producinga burgundy red or red-brown color in the top centimeter of the medium (the bottom portion of the medium remains acidic) ().This reaction can only be detected if lysine decarboxylase is not produced, which is the case with , Morganella species. Indole is produced in this medium by organisms that possess the enzyme tryptophanase.degrades typtophan present in the casein peptone, yielding indole. It can be detected in the medium by adding Kovacs reagentto the agar surface. Indole combines with the p-dimethylaminobenzaldehyde of Kovacs reagent and produces Qdbnlldmcdc enr hcdmshehbashnm ne ldladrr ne nm sgd aarhr ne lnshkhsx, kxrhmd cdbaranwxkard, kxrhmd HiMedia Laboratories Technical Data User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not . Reller L. B. and Mirrett S., 1975, J. Clin. Microbiol., 2:247. . Ewing W. H., 1986, Edwards and Ewings Identification of , 4th Ed., Elsevier Science Publishing Inc., New York, N.Y. . Forbes B. A, Sahm A. S. and Weissfeld D. F., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc., St. . Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical5.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams Revision : / 201 Proteus vulgaris ATCC 50-100 positive, positive positive negative Salmonella ATCC 13076 50-100 positive, negative negative positive Shigella flexneri ATCC 12022 50-100 negative, occasional negative negative User must ensure safe disposal by autoclaving and0or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must HiMedia Laboratories Pvt. Ltd. Reg.office : 33, Vadhani Ind.Est., LBS Marg, Mumbai-500097, Customer care No.: 033-7117 Mumbai-500097, India. Customer care No.: 033-7157 1919 Email: HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Cultures are stab-inoculated and incubated at 37°C for 18-24 hours. Motility, lysine deamination and lysine decarboxylationreactions are read before testing indole reaction, since addition of Kovacs reagent causes the colour of the medium to change adenrd nodmhmf Motility, lysine deamination and lysine decarboxylation reactions are read before testing indole reaction, since addition ofKovacs reagent causes the colour of the medium to change to yellow. Therefore positive lysine decarboxylase reaction Quality ControlAppearanceCream to greenish yellow homogeneous free flowing powderGellingSemisolid, comparable with 0.2% Agar gel.Colour and Clarity of prepared mediumReddish purple coloured clear to slightly opalescent gel forms in tubes as buttsReactionReaction of 3.65% w/v aqueous solution at 25°C. pH : 6.6±0.2pH6.40-6.80Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Motility Indole Lysine Lysine 50-100 positive, negative negative positive Escherichia coli ATCC 25922 50-100 positive, positive, red negative positive Klebsiella pneumoniaeATCC 13883 50-100 negative, occasional negative positive 50-100positive,