Ingredients Gms Litre Peptone 1000 Potassium dihydrogen phosphate 3560 Disodium hydrogen phosphate 7230 Sodium chloride 4300 Final pH at 25 ID: 242001
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Please refer disclaimer Overleaf. Buffered Peptone Water with NaCl M1275 Buffered Peptone Water with NaCl is recommended as a diluent for carrying microbial limit test from clinical and nonclinical specimens.Composition** Ingredients Gms / Litre Peptone 1.000 Potassium dihydrogen phosphate 3.560 Disodium hydrogen phosphate 7.230 Sodium chloride 4.300 Final pH ( at 25°C) 7.0±0.2 **Formula adjusted, standardized to suit performance parameters Suspend 16.09 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Add 0.1 to 1% bs eesisee. and sterilize by autoclaving at 15 lbs pressure (121°C) for Principle And Interpretation Buffered Peptone Water is a pre-enrichment medium designed to help recovery of sub-lethally damaged Salmonellae beforetransfer to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides for resuscitation of the cells that have been injured by processes of food preservation. It was noted by Edel and Cmppe ; Fppe boe ebisz sbnpmes Fps dmioidbm sbnpmes gpmmpw bppsppsibue uedioirues gps iboemiog pes esubbmisiee guieemioes ) HiMedia Laboratories Technical Data Please refer disclaimer Overleaf.Cultural responseCultural characteristics observed after recovery on Soybean Casein Digest Agar after an incubation at 30-35°C for 18-24 Organism Inoculum Recovery Recovery Recovery Escherichia coli ATCC 50 -100 no decrease incolony count no decrease in no decrease in Escherichia coli ATCC 50 -100 no decrease in no decrease in no decrease in Escherichia coli NCTC 9002 50 -100 no decrease in no decrease in no decrease in Staphylococcus aureus 50 -100 no decrease in no decrease in no decrease in Staphylococcus aureus 50 -100 no decrease in no decrease in no decrease in Pseudomonas aeruginosa 50 -100 no decrease incolony count no decrease in no decrease in Quality ControlAppearanceWhite to cream homogeneous free flowing powder Colour and Clarity of prepared mediumclear solution without any precipitate pH Qead sge Pseudomonas aeruginosa 50 -100 no decrease in no decrease in no decrease in Salmonella Typhimurium 50 -100 no decrease in no decrease in no decrease in HiMedia Laboratories Technical Data (stored at2-8°C) Reference 1.Edel and Kampelmacher, 1973, Bull. W.H.O., 48:167.2.Sadovski, 1977, J. Food Technol., 12:85.3.Juven, Cox, Bailey, Thomson, Charles and Schutze, 1984, J. Food Prot., 47:299.4.Angelotti, 1963, Academic Press, New York, N.Y.5.Indian Pharmacopoeia, 1997, Ministry of Health and Family Welfare,Govt. of India, Vol. 2.6.European Pharmacopoeia, 2008, European Directorate For The Quality of Medicine Micrococcus luteus ATCC 50 -100 no decrease in no decrease in no decrease in Candida albicans ATCC 50 -100 no decrease in no decrease in no decrease in Candida albicans ATCC 50 -100 no decrease in no decrease in no decrease in Salmonella Abony NCTC 50 -100 no decrease in no decrease in no decrease in Bacillus subtilis 50 -100 no decrease in no decrease in no decrease in so sge sge produbs. Hmproper ssorage uensikased area Uses nusu eosuse sbge eisppsbm bz buupdmbviog boe0ps iodioesbuipo pg usee ps uousbbme psepbsbuipos pg uiis pspeudu. Fpmmpw esubbmisiee mbbpsbupsz pspdeeuses io eisppsiog pg iogeduipus nbuesibms boe nbuesibm uibu dpnes ioup dpoubdu wiui dmioidbm Dpwoes F. P. boe Iup K.- )Ee.*- 3112- Dpnpeoeiun pg Neuipes gps uie Nidspbipmpgidbm Exbniobuipo pg Fppes- 5ui Anesidbo Pubmid Hebmui Asspdibuipo- Tuboebse Neuipes gps uie Exbniobuipo pg Dbisz Pspeudus- 2989- 25ui Ee.- . Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Revision : 02 / 201 HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: techhelp@himedialabs.com Website: www.himedialabs.com In vitro diagnostic medicaldeviceCE Marking6o not use if package is damaged CE Partner 4U ,Esdoornlaan 13, 3951 DB Maarn The Netherlands, www.cepartner 4u.eu IVD ED REP