Please refer disclaimer Overleaf.Skim Milk AgarM763Skim Milk Agar is u - PDF document

HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negativesurroundingcolonies Escherichia coli ATCC 50-100 good-luxuriant �=70% negativeclear zonesurrounding Proteus mirabilis ATCC25933 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies Pseudomonas aeruginosaATCC 27853 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies Serratia marcescens ATCC8100 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlUser must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positiveclear zonesurroundingcolonies Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Spowder Read the HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui In vitro diagnostic medicalCE Markingnot use if package is ,Esdoornlaan 13, 3951 IVD 24 Wbeiboi Joevtusibm Ftubuf, Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Tbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo Anfsidbo Pvcmid Ifbmui Attpdibuipo, Mfuipet gps uif Fybniobuipo pg Ebisz Pspevdut, 2:78, 25ui Fe/, Phbd D,V,* HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Tbmgiohfs Y/, Upsupsfmmp M/M/ Figui Midspcipmphidbm Fybniobuipo Anfsidbo Pvcmid Ifbmui Attpdibuipo, Tuboebse Mfuipet gps uif Fybniobuipo pg Ebisz Pspevdut, 2:78, 25ui Fe/, Phbd D,V,* Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Tbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Anfsidbo Pvcmid Ifbmui Attpdibuipo, Tuboebse Mfuipet gps uif Fybniobuipo pg Ebisz Pspevdut, 2:78, 25ui Fe/, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Anfsidbo Pvcmid Ifbmui Attpdibuipo, Tuboebse Mfuipet gps uif Fybniobuipo pg Ebisz Pspevdut, 2:78, 25ui Fe/, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the growth of Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negativesurroundingcolonies Escherichia coli ATCC 50-100 good-luxuriant �=70% negativeclear zonesurrounding Proteus mirabilis ATCC25933 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies Pseudomonas aeruginosaATCC 27853 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies Serratia marcescens ATCC8100 50-100 luxuriant �=70% positiveclear zonesurroundingcolonies Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum(CFU) Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positivereaction,clear zonesurroundingcolonies Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories Technical Data Enterococcus faecalis ATCC29212 50-100 luxuriant �=70% negative Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Storage and Shelf LifeStore below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product expiry date on the label.Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product expiry date on the label.Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product expiry date on the label.Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not /MbdFbeeio J/ F/, 2111, Cipdifnidbm Uftut gps Jefouigidbuipo pg Mfeidbm Cbdufsib, 4se Fe/, Mippiodpuu, Wimmibnt boe Wimliot, Wfis I/ M/ boe Fsbol J/ I/, 2115, Tuboebse Mfuipet gps uif Midspcipmphidbm Fybniobuipo pg Ebisz Pspevdut, 27ui Sfwitipo : 14 0 2127 HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com Mbpbhf T/, Tifmupo J/ boe Miudifmm 2:71, Mfuipet io Midspcipmphz', Npssit J/ boe Siccpot E/, (Fet/), Wpm/ 4A, der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). Phbd D,V,* 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der GesamtkeimzahlTbmgiohfs Y/, Upsupsfmmp M/M/ Figui Mfuipet gps Midspcipmphidbm Fybniobuipo Pspdfevsft Iboec1ppl/ 2 HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the opening the be referred in 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positivereaction, Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by(2) for cultivation and enumeration of microorganisms encountered in dairy industry (3). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (4). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the before opening handling specimens 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57.3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,4.Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl Figui (Fe/), Pspdfevsft Iboec1ppl/ 2 HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57. . . Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., . Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl Figui (Fe/), Pspdfevsft Iboec1ppl/ 2 HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by) for cultivation and enumeration of microorganisms encountered in dairy industry (). Addition of SM powder to anynutrient-rich medium creates favourable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the before opening handling specimens 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by) for cultivation and enumeration of microorganisms encountered in dairy industry (). Addition of SM powder to anynutrient-rich medium creates favorable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the before opening handling specimens 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by) for cultivation and enumeration of microorganisms encountered in dairy industry (). Addition of SM powder to anynutrient-rich medium creates favorable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species.2.Some strains show less growth due to variable nutritional repuirements, HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. the product. the product Use before expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended by) for cultivation and enumeration of microorganisms encountered in dairy industry (). Addition of SM powder to anynutrient-rich medium creates favorable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57. . . Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., . Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl Figui (Fe/), HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20Cultural ResponseM763: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. formation due the hygroscopic the product. the product dry ventilated Use before expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm Please refer disclaimer Overleaf.M763 SMAgar Ingredients Gms / Litre Spowder 28.000 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25°C) 7.0±0.2**Formula adjusted, standardized to suit performance parameters Suspend 51.5 grams of in 1000 ml water. Heat to boiling to dissolve the medium Principle And Interpretation SM Agar is used for the demonstration of coagulation and proteolysis of casein (1). The medium is recommended byfor cultivation and enumeration of microorganisms encountered in dairy industry (). Addition of SM powder to anynutrient-rich medium creates favorable conditions for growth of organisms, which are encountered in milk. The number ofbacteria isolated thus is more than the number of organisms isolated on a regular medium (). Proteolytic bacteria hydrolyzecasein to form soluble nitrogenous compounds indicated as clear zone surrounding the colonies. More clear zones are seen on provides amino acids and other complex nitrogenous substances. Yeast extract supplies vitamin BAddition of SM powder in the medium makes the conditions optimal for microorganisms encountered in milk. Glucose Spowder Read the 1.Further biochemical identification is repuired for identification of species. HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086,India Customer care No.: 022-6116 9797Corporateoffice : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:techhelp@himedialabs.com Website: www.himedialabs.com der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57. . . Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., . Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl Figui (Fe/), HiMedia Laboratories Technical Data Enterococcus faecalis ATCC 29212 50-100 luxuriant �=70% negativereaction, no Escherichia coli ATCC 25922 50-100 good-luxuriant �=70% negative Proteus mirabilis ATCC 50-100 luxuriant �=70% positive Pseudomonas aeruginosa 50-100 luxuriant �=70% positive Serratia marcescens ATCC 50-100 luxuriant �=70% positive Colour and Clarity of prepared mediumOff white coloured opaque gel forms in Petri platesReactionReaction of 5.15% w/v aqueous solution at 25°C. pH : 7.0±0.2pH6.80-7.20 Cultural ResponseCultural characteristics observed after an incubation at 35-37°C for 18-24 hours. Organism Inoculum Growth Recovery Proteolytic Bacillus subtilis ATCC 6633 50-100 good-luxuriant�=70% positive Quality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with 1.5% Agar gel Please refer disclaimer Overleaf. formation due the hygroscopic the product. the product dry ventilated Use before expiry date on the label. Vtfs nvtu fotvsf tbgf eitpptbm cz bvupdmbwioh boe0ps iodiofsbuipo pg vtfe ps vovtbcmf psfpbsbuipot pg uiit pspevdu/ Fpmmpx ftubcmitife mbcpsbupsz pspdfevsft io eitpptioh pg iogfduipvt nbufsibmt boe nbufsibm uibu dpnft ioup dpoubdu xiui dmioidbm HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967). 1.Frazier W. C. and Ripp P., 1928, J. Bacteriol., 16: 57. . . Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., . Terplan G. Rundfeldt,H.u. Zaadhof, K.J. Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl Figui (Fe/),