Ingredients Gms Litre Casein enzymic hydrolysate 10000 Potassium nitrate 1000 Mannitol 7500 Phenol red 0040 Agar 3500 Suspend 2204 grams in 1000 ml distilled water Heat to boiling to dissolve ID: 381442
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Please refer disclaimer Overleaf.Mannitol Motility Nitrate MediumM1320Mannitol Motility Nitrate Medium is used for studying mannitol fermentation, nitrate reduction and motility of bacteria.Composition** Ingredients Gms / Litre Casein enzymic hydrolysate 10.000 Potassium nitrate 1.000 Mannitol 7.500 Phenol red 0.040 Agar 3.500 Suspend 22.04 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense into test tubes.Mannitol Motility Nitrate Medium is designed to differentiate bacteria on the basis of their motility, ability to ferment mannitoland reduce nitrate (1). The highly nutritious casein enzymic hydrolysate supports luxuriant growth of bacteria. Semisolid natureof the medium due to 0.35% agar helps to detect motility. Motile bacteria produce diffused growth throughout the mediumwhile non motile bacteria grow only along the line of inoculation. Combination of mannitol and phenol red helps differentiationReduction of nitrate is generally an anaerobic respiration in which an organism derives its oxygen form nitrate. Members of characteristically reduce nitrate to nitrite which reacts with sulfanilic acid and dimethyl-1-napthylamine Organism Growth Mannitol Motility Nitrate Cultural Response Escherichia coli ATCC luxuriant Positive Positive, Positive HiMedia Laboratories Technical Data Proteus vulgaris ATCC13315 luxuriant Negative Positive, Positive Salmonella Typhi ATCC luxuriant Positive Positive, Positive Shigella sonnei ATCC 25931 luxuriant Positive Negative,stabline,surroundingmedium Positive Staphylococcus aureus luxuriant Positive Negative, Positive Staphylococcus epidermidis luxuriant Negative Negative, Positive User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not