PDF-YT Agar is recommended for growth of

Ingredients Gms Litre Casein enzymic hydrolysate 8000 Yeast extract 5000 Sodium chloride 5000 Agar 10000 Suspend 2800 grams in 1000 ml distilled water Heat to boiling to dissolve the medium co. A medium is sterilized (living organisms removed) before usage in the lab. . Sterilization . methods . include:. . Autoclaving.. Dry-heat.. Filtration.. UV . exposure . and . ethylene oxide. . Culture:. By. : Dr. . S. S. . Khoramrooz. Department of Microbiology, Faculty of Medicine, . Yasuj. University of Medical Sciences, . Yasuj. , Iran. Yasuj. University of Medical Sciences. Department of Microbiology. Gms / Litre 15.000 5.000 Agar HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Recovery Growth w/ Recovery w/ Cultural Response Growth at 30-35 SELMA ABDUL SAMAD. BCH-10-05-02. S1-MSc.BIOCHEMISTRY. NUTRITIONAL REQUIREMENTS. OF BACTERIA. Nutrients . are substances used in biosynthesis and energy production. Hence required for . bacterial growth. Ingredients Gms / Litre Heart Infusion solids 10.000 Proteose peptone 10.000 Lactose 10.000 Sodium deoxycholate 5.000 Neutral red 0.020 Sodium citrate 20.000 Ferric ammonium citrate 2.000 Agar 13.500 in bacteriology labs. - Definition. - History. - Main aims. - Rate of solidity. Agar. composed . of two long-chain polysaccharides (. 70% . agarose. . and. 30. % . agarapectin. ). . melts . at 95. o. Takes a very long time. Uses a colorimeter. Requires very simple apparatus. Need to use a calibration curve. Counts only live cells. Counts dead and live cells. L2 – Bacterial Growth. Understand . the different phases of a bacterial growth curve (lag phase, log phase, stationary phase and death phase) and calculate exponential growth rate constants. Lab Pro pp . 235-277. Reference: Laboratory Procedures for Veterinary Technicians 5. th. . ed. (Hendrix & . Sirois. ). Microbiology: The study of microbes. ___________________. : organisms too small to be seen with the naked eye. FOODS. SAMPLING . Sampling Plans. Decisions have to be made, prior the counting, as to how many sample of the product should be examined, what size sample should be taken for analysis, and what method should be used for sampling the product. Vol. 5(25 ) , pp. 4321 - 4327, 9 November , 2011 Available online at http://www.academicjournals.org/ AJMR ISSN 1996 - 0808 Ingredients Gms / Litre L-Glutamic acid 0.500 DL-Alanine 0.500 Asparagine 0.500 Dextrose 10.000 Dipotassium phosphate 15.000 Monopotassium phosphate 5.000 Ammonium chloride 2.500 Ammonium nitrate 0.50 E. ndospores staining; Microbial culture media and sterilization; Methods of inoculation and isolation of pure culture.. Asst. Lec. Mariam A. Ali. Al-Mustaqbal University college 2 . Equipments. Department of Microbiology . College of Medicine. Knowing the microscopic morphology and staining characteristics of a microorganism present in a clinical specimen, the microbiologist can make appropriate decisions as to how it should be cultivated and what biologic properties must be demonstrated to identify it fully.. Case #1. According to the history provided, a 10 year old female spayed Dalmatian presented with a 5-day history of anorexia and . polydipsia. , several weeks of decreased appetite, and multiple episodes of vomiting and weight loss (5 pounds) in...