PPT-Blood Agar

Biology amp Biotechnology Department PURPOSE Blood agar is used for isolation and cultivation of many types of fastidious bacteria It is also used to differentiate bacteria based on their hemolytic characteristics especially within the genera . A medium is sterilized (living organisms removed) before usage in the lab. . Sterilization . methods . include:. . Autoclaving.. Dry-heat.. Filtration.. UV . exposure . and . ethylene oxide. . Culture:. By. : Dr. . S. S. . Khoramrooz. Department of Microbiology, Faculty of Medicine, . Yasuj. University of Medical Sciences, . Yasuj. , Iran. Yasuj. University of Medical Sciences. Department of Microbiology. & . Identification. of . Staphylococci. بسم الله الرحمن الرحيم. Staphylococci Characteristics. Staphylococci are often found in the human nasal cavity (and on other mucous membranes) as well as on the skin. . monocytogenes. Basmah. . almaarik. #Lab 7. Listeria. spp.. Listeria. . monocytogenes. . is the only species that infect humans.. Infection most common in:. Pregnant women .  still birth. Fetuses . Electrophoresis. Electrophoresis. Electrophoresis is a means of separating hemoglobin's. . . It depends on the migration of the hemoglobin molecules dissolved in a buffer on, or in, a supporting medium when an electric current is passed through them.. in bacteriology labs. - Definition. - History. - Main aims. - Rate of solidity. Agar. composed . of two long-chain polysaccharides (. 70% . agarose. . and. 30. % . agarapectin. ). . melts . at 95. o. Staph. , . Strep. and Gram – pathogen part II. LAB . # . 12. Medgar Evers College. Prof. Santos. Exercise 70 week 3. Last week you streaked 2 plates, SM110 and MSA. Today, you will pick isolated colonies from the plates that are characteristic of . Biology & Biotechnology Department. PURPOSE :. Blood agar . is . used for . :. isolation and cultivation of many types of fastidious bacteria. . It is also used to differentiate bacteria based on their hemolytic characteristics, especially within the genera . Dr . maryam. . Sotoudeh. . Tehran heart Center. 1. Categories of Microbiological Media. Media . are divided into two main descriptive . categories based on the . purposes. for which the media were designed : . Ingredients Gms / Litre L-Glutamic acid 0.500 DL-Alanine 0.500 Asparagine 0.500 Dextrose 10.000 Dipotassium phosphate 15.000 Monopotassium phosphate 5.000 Ammonium chloride 2.500 Ammonium nitrate 0.50 Part II. MJ. Weintraub, . DVM. Cell wall/envelope structure . GN. , GP, AF & wall-less. Genome . Morphology, arrangements, capsule/slime layer, flagella, fimbriae, . pilus. Horizontal gene transfer mechanisms. Method of specimen collection. Lumbar puncture (LP), . is . a medical procedure in which a needle is inserted into the . spinal . canal. .. . most commonly to collect . cerebrospinal fluid.  (CSF) for diagnostic testing. . Required to isolate the bacteria from the clinical specimens.. . CONSTITUENTS OF CULTURE MEDIA. Constituent. Explanation. 1)Water. Distilled water or potable water with low mineral content.. Serves as the source of hydrogen and oxygen. . Case #1. According to the history provided, a 10 year old female spayed Dalmatian presented with a 5-day history of anorexia and . polydipsia. , several weeks of decreased appetite, and multiple episodes of vomiting and weight loss (5 pounds) in...