PDF-Proc.Natl.Acad.Sci.USAVol.90,pp.6275-6279,July1993ImmunologyRoleofthei

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ProcNatlAcadSciUSA901993solvedinapyrogenicwaterandthesolutionwasfilteredthrougha022mcelluloseacetatefilterThelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof147mgmlLipopoly

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Proc.Natl.Acad.Sci.USAVol.90,pp.6275-6279,July1993ImmunologyRoleofthei: Transcript

ProcNatlAcadSciUSA901993solvedinapyrogenicwaterandthesolutionwasfilteredthrougha022mcelluloseacetatefilterThelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof147mgmlLipopoly. Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA92(1995)7141FRETefficiencyRNAbulgeA7AgFIG.2.KinkingofDNAandRNAduplexesbybasebulgesisrevealedbyFRET.Thedistancebetweendonor(D)andacceptor(A)fluorophoresattachedtothe5'terminiofthe Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA92(1995)11999BacterialcellEukaryoticcellCalmodulinActive*-*tJ~'X-cyclaseATPcAMPFIG.1.Strategytoanalyzeinternalizationofbacterialproteinsinsidethecytosolofeukaryoticcells.sec,Gene Proc.Natl.Acad.Sci.USA82(1985)ofcellsoveriodinevapors.Cellsthataccumulateglycogenstainbrown,whereasthosethatdon'tremainyellow.Quan-titativetrehaloseandglycogendeterminationsweremadeaccordingtoLilliean Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest

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