PPT-Purification Techniques 1

Introduction The organization of this presentation includes I Basic methods of Isolation and purification II Special Methods of purification a Countercurrent Extraction. 43. Copyright © McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display.. Water Purification and . Sanitary Analysis. Microbial . containment candidates. potential pathogens that can survive in water and represent severe health risks. Ian Gluck. Mentor: Dr. Christine Kelly. OSU Dept. of Chemical, Biological and Environmental Engineering. The Biofuel Industry. The Conversion Process. Cellulose must be converted to glucose. Performed by cellulases. – Sudan , . Flat . No 3 . building N 612, block 4 , Hay . Alsafa. . Tel. : +249999899986 - +249123004703 . Email. : . hussein39@gmail.com. . HRS . Oy. : . St. . Petersburg Russia, E-mail: . Course: Methods in protein chemistry. Rahman M. . Mahfuz. ur. 2012/01/11. SLU. To seperate a . particular. protin from all other proteins and cell . components. . There are many types of proteins within an ogranism . HIC Chromatography. Chromatography. A technique used to separate molecules based on how they tend to cling to or dissolve in various solids, liquids, and gases.. Characteristics of Proteins. Hydrophilic. RECORDINGMICROPHONE TECHNIQUES 3 Microphone Techniques.............4..................................................................................5Microphone Techniques ........................... Plasmid . What is the plasmid ?. Plasmid are small, double strand , closed circular DNA molecules , that can be isolated from . bacterial cells. , they replicate independently of the bacterial chromosome. – Sudan , . Flat . No 3 . building N 612, block 4 , Hay . Alsafa. . Tel. : +249999899986 - +249123004703 . Email. : . hussein39@gmail.com. . HRS . Oy. : . St. . Petersburg Russia, E-mail: . Proposing a Procedure. Library. Primary literature. SciFinder. Scholar. Secondary Literature. Organic Syntheses. Fieser. Comprehensive Organic Transformations. Case Study: Benzoic Acid. Literature: Try a Grignard Reaction. All pupils will be able to (Baseline): . Identify the different purification techniques.. Most pupils will be able to (Further): . Investigate which purification technique is most effective.. Some pupils will be able to (Challenge. Part I. IUG, Fall 2012. Dr. . Tarek. . Zaida. 1. Background. LDH catalyzes the . nicotinamide. cofactor-dependent . interconversion. of lactate and . pyruvate. :. 2. LDH is found in almost all organisms because it plays an important role in carbohydrate metabolism during conditions in which . Iman . Alshehri. Introduction . Iman . Alshehri. In this course, you will use lactate dehydrogenase (LDH) as the subject of . your studies. . LDH (. E.C. . 1.1.1.27) catalyzes the nicotinamide . cofactor-dependent. TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an . Many cloning vectors have been engineered so that. the protein being expressed will be fused to another. protein, called a . tag, which can be used to facilitate. protein purification. Examples of tags include glutathione-.