RibU Jianing Song sjnskhotmailcom 20141206 Membrane protein other protein 25 Membrane protein Category of membrane protein Focus on 1 Membrane receptor signal transduction GPCR 2 membra ID: 851013
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1 Unveiling the gating mechanism of ECF Tr
Unveiling the gating mechanism of ECF Transporter RibU Jianing Song sjn_sk@hotmail.com 2014.12.06 Membrane protein other protein ~25% Membrane protein Category of membrane
2 protein Focus on: 1, Membrane receptor
protein Focus on: 1, Membrane receptor: signal transduction, GPCR 2, membrane enzymes: activity, r - secretase 3, Ion channel: ions flow, Na+/K+ pump 4, Membrane transp
3 orter: molecules, ECF - transporter GPC
orter: molecules, ECF - transporter GPCR S A ECF - transporter Micronutrients Energy - Coupling - Factor Transporter S Component: binding molecules T Component: not clear A
4 ,A’ Component: binding ATP Energy - Co
,A’ Component: binding ATP Energy - Coupling - Factor Transporter VB2 VB1 Structure of S component Zhang, P.; Wang, J. & Shi, Y. Nature , 2010, 468, 717 - 720 S compone
5 nt RibU S tatic structure analysis: 1,
nt RibU S tatic structure analysis: 1, L1 is used to control the binding of VB2. 2, VB2 is transported through RibU Structure for simulation Yellow and green: lipid bilay
6 ers Blue : membrane protein Gray : water
ers Blue : membrane protein Gray : water Package: NAMD Time length: 180 ns Results VB2 binding The conformational changes occurs to L5’. Initially, it is open, but afte
7 r VB2 binds, it closes. It is regarded t
r VB2 binds, it closes. It is regarded that L5’ serves as a gate c ontrolling the binding. Jianing Song, Changge Ji , John Z. H. Zhang (2013). Scientific Reports. 356
8 6 Results As vitamin B2 comes near to t
6 Results As vitamin B2 comes near to the binding pocket, L5’ closes slowly and slowly. L5’ binding mechanism Jianing Song, Changge Ji , John Z. H. Zhang (2013). Scie
9 ntific Reports. 3566 Results Left hand
ntific Reports. 3566 Results Left handle: TM1, TM2, TM3 Right handle: TM4, TM5, TM6 Force the two handles away from each other Transport mechanism Jianing Song, Changge J
10 i , John Z. H. Zhang (2013). Scientific
i , John Z. H. Zhang (2013). Scientific Reports. 3566 Results movie Green: lipid molecule Purple: RibU Lipids go through the pulled channel and prevent the transporting
11 of VB2 Transport mechanism Jianing Son
of VB2 Transport mechanism Jianing Song, Changge Ji , John Z. H. Zhang (2013). Scientific Reports. 3566 Conclusion 1 , L 5 ’ is a gate controlling the binding of Vita
12 min B 2 , not L 1 . 2 , RibU alone is no
min B 2 , not L 1 . 2 , RibU alone is not able to transport riboflavin through lipid bilayers The transport channel should be between S component and T component . Thank you