MENT OF EFFICIENCY OF ETHANOL PRODUCTION FROM JERUSALEM ARTICHOKE Quang D Nguyen Kálmán Dénes Csilla Farkas Ágoston Hoschke Judit M RezessySzabó T he 2 ID: 526427
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ENHANCEMENT OF EFFICIENCY OF ETHANOL PRODUCTION FROM JERUSALEM ARTICHOKE
Quang D. Nguyen, Kálmán Dénes, Csilla Farkas, Ágoston Hoschke, Judit M. Rezessy-Szabó
T
he 2
n
d
International Symposium „Vera
Johanides
“ 2013 Slide2
Production of bioethanol in EU-27 - 2009
(million liters)Source: www.biofuels-platform.ch
Welcome
Croatia
to
be
in
this map (EU-28)! On the 3rd International Symposium „Vera Johanides“ in 2015 – Biotechnology and Forestry Complex in Croatia?
Total: 3703 Slide3
Productivity of ethanol using different raw materials
Plant
Main carbohydrate
Productivity
(l/
ha)
Sugarcane
saccharose
8000
Sugar
beet
saccharose
3300
Jerusalem artichoke
inulin
4200
corn
starch
2100
potato
starch
1900
wheat
starch
1800
wood
cellulose/lignocellulose
?????Slide4
Potential of JA-based bioethanol production
Jerusalem artichoke is easy to cultivateRelatively high production yields: 16–20 t/ha (even 20-80 t/ha) for tubers, and 18–28 t/ha green weight for foliageHigh carbohydrate content: 15-20 % mainly inulin and fructo-oligosaccharidesCultivate yearlyCan be harvested like potatoSlide5
Problems addressedSaccharomyces cerevisiae
strain does not exhibit so high inulinase activityTreatment: acidic or enzymaticExogenous enzyme preparationLow efficiency of bioconversion (60-70 %)Slide6
ObjectivesTechnolog
ical development for enhancement of efficiency of ethanol production using Jerusalem artichoke as raw material
Inoculation technique
Simultan saccharification and fermentation
Semi-continuous fermentation
technologySlide7
Development of inoculation technique
Effects of various inoculation techniques were investigated Best one
on initial
day 2/3 amount of inoculum (
cell number) and on
second
day
: 1/3
amountEfficiency of bioconversion: 90 % Slide8
SSF vs. Mixed Culture Fermentation
Efficiency of bioconverion: 85-88 %Slide9
Semi-continuous technology
Fresh mash
Harvested
Fresh mixed cultureSlide10
ConclusionThe use of mixed culture
in combination with semi-continuous fermentation technology definetely increases in the productivity,
thus it is
very promising for development of technology for production of bioethanol based on Jerusalem artichoke as raw
materialSlide11
AcknowledgementThis work was supported by Hungarian Development Agency through Project number
TÁMOP-4.2.1./B-09/1-KMR-2010-0005 and TECH_09-A3-2009-0194, as well as Hungarian Academy of Science through Bolyai János Research GrantSlide12
Thank you for your kind attention!