Ligninase Termostable Produced from Thermophilic Bacteria Yetti Marlida Department of Animal Nutrition and Feed Technology Faculty of Animal Science Andalas University Indonesia ID: 795267
Download The PPT/PDF document "The Fiber Fraction of Oil Palm Trunk T..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
The
Fiber Fraction of
Oil Palm Trunk Treated by
Ligninase
Termostable
Produced
from
Thermophilic
Bacteria
Yetti Marlida
Department
of Animal Nutrition and Feed Technology, Faculty of Animal Science,
Andalas
University
, Indonesia
Slide2Map of Indonesia
Slide3Background of Research
Indonesia is 2 nd country after Malaysia produced oil palm in the world
The main biomass of oil palm industry are :
OPF, OPT
,
PPF,
PKC and POME
– as sources as animal feeding
OPT is only available after oil palms are felled for replanting at an age of about 25 years.
OPT
are high fibrous materials which need further processing for better utilization in ruminant animals
(lignin, cellulose and hemicellulose)
Slide4Background of Research
The OPT : lignin, cellulose, hemicellulose – complexs structure difficult to attack by MO
As animal feeding : OPT- 3 limiting Factors (Lignin protects of cellulose, hemicellulose: Low palatability: low protein content )
Lignin is indigestible by animal enzymes, but some fungi and bacteria are able to secrete ligninases which can biodegrade of the polymer
Slide5Purpose of Research
To find out interaction between oil palm trunk concentrations and ligninase doses to increase the fiber fractions of oil palm trunk before using as animal feeding
To get the future treatment of how to increase the palm oil trunk quality
Slide6Method of Research
Slide7Research finding
A
[Ligninase (U/kg)]
B
[oil palm trunk (%)]
Average
B1
B2
A1
64.00
a
64.16
a
64.08aA262.31b64.44a63.38aA360.75c61.54bc61.15bAverage62.35b63.38a
A [Ligninase (U/kg)]B [oil palm trunk (%)]AverageB1B2A180.97b77.18c79.07bA281.58a81.80a81.69aA376.68d76.76d76.72cAverage79.74a78.58b
Table 1. Average ADF products hydrolysis of oil palm trunks (%)
Table
2
.
Average
NDF
products hydrolysis of oil palm trunks (
%
)
Slide8Research finding
A[Ligninase (U/kg)]
B[oil palm trunk (%)]
Average
B1
B2
A1
16.98
b
13.02
d
15.00
b
A2
19.27
a17.36b18.32aA315.93c15.21c15.57bAverage17.39a15.20a
A [Ligninase (U/kg)]B [oil palm trunk (%)]AverageB1B2A156.41b55.38bc64.08aA254.13c58.07a63.38aA354.06c52.43d61.15bAverage54.87a
55.29aTable
3
.
Average
ADF
products hydrolysis of oil palm trunks (%)
Table
4
.
Average
NDF
products hydrolysis of oil palm trunks (
%
)
Slide9A
[Ligninase (U/kg)]
B [oil palm trunk (%)]
Average
B1
B2
A1
7.31
b
7.23
bc
7.27
a
A2
7.09c5.60e6.35cA36.07d7.67a6.87bAverage6.82a6.83a
Research findingTable 5. Average ADF products hydrolysis of oil palm trunks (%)
Slide10Conclusion
The treated of OPT with ligninase can be degraded the lignocellulose and hemicellulose linkage and make the availability of cellulose and hemisellulose as energy sources by ruminantia such as cow, buffalo, sheep and goat. The optimum concentration of oil palm trunks was 60% (v/w) and 750 U/kg of ligninase thermostable that improved of the fiber fractions quality to easily digested by animal.
Slide11Acknowledgement
Ministry of Research, Technology and Higher Education of the Republic of Indonesia
for funding this research
Student and technician laboratorium of Industrial and Feed Technology who has supported the implementation of this research.
Slide12Thank You for your attention