PDF-OURNALOTANY[Vol. 99 0.2mm) of solid growth medium (1% agarose), and

MM MATERIALS AND METHODS Sample preparation. A medium is sterilized (living organisms removed) before usage in the lab. . Sterilization . methods . include:. . Autoclaving.. Dry-heat.. Filtration.. UV . exposure . and . ethylene oxide. . Culture:. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous.  . Orderly increase in the sum of all the components of an organism, cell multiplication is a sequence of growth; in unicellular organism, growth leads to an increase in the number of an individuals making up a population or culture.. in bacteriology labs. - Definition. - History. - Main aims. - Rate of solidity. Agar. composed . of two long-chain polysaccharides (. 70% . agarose. . and. 30. % . agarapectin. ). . melts . at 95. o. Presentation by: . Mr. . Loi. M. Bakani CMG. Governor-Bank of PNG. National . D. evelopment . F. orum. 18. th. October 2016 . Port Moresby. Presentation Parts. Background . of current financial and economic issues in PNG. Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. 1,000,000,000. PCR cycles. 20. 40. 30. Theorectical. yield. Actual yield. “Plateau effect”. No. of DNA copies. How much DNA amplification has occurred?. How do scientists analyse these PCR products?. One of the basic tools of modern biotechnology is gene splicing.. This is the process of removing a functional DNA fragment ( a gene) from one organism and combining it with the DNA of another organism to study how the gene works.. BCH 333 [practical]. Lab# 8. Objective:. -To be familiar with Agarose gel electrophoresis principle. . Agarose gel electrophoresis:. is a method of gel electrophoresis used in biochemistry and molecular biology to separate and analyze DNA or RNA molecules by size.. Objective: To visualize pieces of DNA by size, using a gel matrix and an electrical current. Background The chromosomes in our cell nuclei consist of large strands of DNA. These strands are very u Casein Enzymic Hydrolysate5.10L-Asparagine5.10Potato Flour 36.40Skim Milk 100.00Malachite Green 1.20Final pH (at 25C) 7.0 0.2Precautions and DisclaimerFor laboratory use only. Not Growing Media . Growing medium The material in which plants grow in pots is known as ‘potting material’, while the substrate or medium used to grow plants is called ‘growing medium’.. The choice of the type of potting material is important as the growth of plants largely depends on it. Harvesting . Gelidium . red algae in Indonesia. Agar and agarose are derived from the cell walls of red algae. Agar is a mixture of agarose and agaropectin. Agarose gel electrophoresis. agar-agar. Malay name for red algae. TOPIC: ELECTROPHORESIS. HANDLED BY: RM.LAKSHMANAN. ASSOCIATE PROFESSOR & HEAD. DEPARTMENT OF MICROBIOLOGY. HAJEE KARUTHA . ROWTHER HOWDIA . COLLEGE(AUTONOMOUS), . UTHAMAPALAYAM . ELECTROPHORESIS.