PPT-Molecular cloning overview

Steps to prepare vector 1 1216 hour overnight culture 2 Minipreps day immediately following ON use Qiaprep spin miniprep kit Measure DNA conc 3 Largescale restriction enzyme digest. (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . Molecular Cloning (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . In biotechnology, cloning refers to the different processes used for . duplicating . biological material . (ex. DNA fragments, cells or organisms). . . The 3 Types of Cloning Technologies:. 1. Recombinant DNA Technology/DNA Cloning. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . technology similar . to artificial insemination, . describe the production of natural clones in plants using the example of vegetative propagation in elm trees; . describe the production of artificial clones of plants from tissue culture;. discuss the advantages and disadvantages of plant cloning in agriculture (HSW6a, 6b, 7c); . pUC18 Product Information Sheet # V330 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC18 high c assisted breeding in animal husbandry. Cloning animals is a reliable way of reproducing superior livestock genetics and ensuring herds are maintained at the highest quality possible. It’s importa AND ITS ETHICAL ISSUES Early Successes - Human Cloning  2001 – First cloned human embryos (only to six cell stage) created by Advanced Cell Technology (USA)  2004* – Claim of first h DEFINITION. HISTORY. STEPS . PROCEDURE. TYPES . EXAMPLES . ADVANTAGES AND DISADVANTAGES. WORD ORIGINATION. The term clone, invented by J. B. S. Haldane, is derived from the Ancient Greek word "twig", referring to the process whereby a new plant can be created from a twig.. HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. .