PPT-1 Cloning Vectors Vector

A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organismHost cell Also referred as carrier of DNA sequence Also referred as cloning vehicle or cloning DNA. A . scalar. quantity . can be described by a . single number. , . with some meaningful . unit. 4 oranges. 20 miles. 5 miles/hour. 10 Joules of energy. 9 Volts . Vectors and scalars. A . scalar. quantity . Any vector can be resized by multiplying it by a real number (scalar).. Multiplying by positive scalar changes magnitude only.. Multiplying by a negative scalar changes the magnitude and its direction.. Jasmin . Sutkovic. Principles . of cloning, vectors and cloning strategies. by . Jasmin SUTJOVIC. Principles of cloning, vectors and cloning strategies. DNA CLONING. DNA cloning is a technique for reproducing DNA fragments. . Mammalian Cells. Gang Zhang, Ph. D. Research Technician II. Centre for Research in Neurodegenerative Diseases. Department of Medicine. University of Toronto, Canada. This talk based on the following publications:. Principle, Requirements, steps and applications Dr. Sudha Kumari Department of Veterinary Microbiology Bihar Veterinary College, Patna Bihar Animal Sciences Un pUC 1 18 Product Information Sheet # V333 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC 1 18 pUC18 Product Information Sheet # V330 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC18 high c Ge cells by methods that pass itacross the membrane naturally, such as a viral vector (in thesa punctures the membrane at veryhigh velocity.Each restriction enzymecleaves a specific targetsequence(usu II Semester Pa per: Genetic Engineering , Genomics and Proteomics By: Professor Bhumi Nath Tripathi (Whatsapp : 9694218853 ; Email : bhuminath.tripathi@igntu.ac.in ) Stay at home, stay safe. Your DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .