PPT-Phage Vectors Bacteriophages

or phages are viruses that specifically infect bacteria Viral vectors are those in which gene of interest is incorporated in the genome of virus The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell. . . Prepare phage for testing. *Create high titer lysate via flooding method.. *Isolate phage DNA and replicate using PCR.. *Send off isolated phage to HHMI facility for . A.S. 1.3.1 – 1.3.4. Scalar Quantities. Those values, measured or coefficients, that are complete when reported with only a magnitude. Examples:. . the table is 2.5 m long. . He ran the 100. m race in 12.65 s.. Which of the following is the odd one out?. Mass. Speed. Force. Temperature. Distance. Elephant. Which of the following is the odd one out?. Mass. Speed. Force. Temperature. Distance. Elephant. Scalars. Objectives. :. Distinguish between vector and scalar quantities. Add vectors graphically. Scalar. – a quantity that can be completely described by a number (called its magnitude) and a unit.. Ex: length, temperature, and volume. Is a . number. with units. It can be positive or negative.. Example: distance, mass, speed, Temperature…. Chapter 3: . VECTORS. 3-2 . Vectors and Scalars. Scalar. Is a quantity with both . direction. How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Motivating Question. : An Airplane flies north with an airspeed of 575 mph. If the wind is blowing 30° north of east at 50 mph, what is the speed of the plane as measured from the ground? What if the wind blew south of west?. By Monique Hunter. Bacteriophages or phages are viruses that infect bacteria.. In 1917, . French-canadian. microbiologist Felix . d’Herelle. observed an unknown substance killing bacteria as did his . Any vector can be resized by multiplying it by a real number (scalar).. Multiplying by positive scalar changes magnitude only.. Multiplying by a negative scalar changes the magnitude and its direction.. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. . . Vaid. , B.C. . Bera. , Sanjay . Barua. , . Riyesh. T. and Praveen . Malik. ). Veterinary Type Culture Collection, . National Research Centre on Equines, . Sirsa. Road, . Hisar. , Haryana, India. A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a .