PPT-CLONING VECTORS Dr. S.

Rubanraj Dept of Mathematics St Josephs College Trichy Vectors Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication If it is used only for reproducing the DNA fragment it is called a . Dr. E. What is a plasmid?. Small double stranded circular DNA. Naturally found in bacteria. It is completely separate form bacterial chromosomal DNA. It independently self replicates . May give bacteria a . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. describe the production of natural clones in plants using the example of vegetative propagation in elm trees; . describe the production of artificial clones of plants from tissue culture;. discuss the advantages and disadvantages of plant cloning in agriculture (HSW6a, 6b, 7c); . A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Jasmin . Sutkovic. Principles . of cloning, vectors and cloning strategies. by . Jasmin SUTJOVIC. Principles of cloning, vectors and cloning strategies. DNA CLONING. DNA cloning is a technique for reproducing DNA fragments. . Chapter 5: Investigating DNA. Major Topics. 5.1 DNA Isolation,  Sequencing, and Synthesis. 5.2 Bioinformatics. 5.3 Cloning and Recombinant Expression. 5.4 Microarrays. 5.5 In Situ Hybridization. Foundations of Biochemistry. Mammalian Cells. Gang Zhang, Ph. D. Research Technician II. Centre for Research in Neurodegenerative Diseases. Department of Medicine. University of Toronto, Canada. This talk based on the following publications:. Principle, Requirements, steps and applications Dr. Sudha Kumari Department of Veterinary Microbiology Bihar Veterinary College, Patna Bihar Animal Sciences Un II Semester Pa per: Genetic Engineering , Genomics and Proteomics By: Professor Bhumi Nath Tripathi (Whatsapp : 9694218853 ; Email : bhuminath.tripathi@igntu.ac.in ) Stay at home, stay safe. Your DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . M13. , f1 and . fd. are filamentous . coliphages. . containing a . circular . single-stranded DNA molecule. . These . coliphages. . have . been developed as cloning . vectors, for . they have a . number . A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .