PPT-On Lentiviral Vector Cloning, Titration, and Expression in

Mammalian Cells Gang Zhang Ph D Research Technician II Centre for Research in Neurodegenerative Diseases Department of Medicine University of Toronto Canada This talk based on the following publications. Human Reproductive Cloning. Is human reproductive cloning (HRC) morally different from natural pregnancy?. In . 2009, . Obama claimed human . reproductive cloning (HRC) is "dangerous, profoundly wrong, and has no place in our society, or any society. Lab 9. Practical . Blood Bank. Titration is a semi quantitative method used to determine the concentration of antibody in a serum sample. or . to compare the strength of antigen expression on different red cell samples. . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Dr. E. What is a plasmid?. Small double stranded circular DNA. Naturally found in bacteria. It is completely separate form bacterial chromosomal DNA. It independently self replicates . May give bacteria a . Lab 9. Practical . Blood Bank. Titration is a semi quantitative method used to determine the concentration of antibody in a serum sample. or . to compare the strength of antigen expression on different red cell samples. . acetic acid, CH. 3. COOH, . pKa. = 4.8. 1.0. 0.8. 0.6. 0.2. 0.4. OH. -. equivalents. pH:. A. H. A. -. H. +. [A. -. ] . : [HA] = 1 : 1000. pH: . 1.8. = . pK. a. –3.0. Titration of a weak acid. acetic acid, CH. A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. 2009, Prentice-Hall, Inc.. Titration. In . this technique a known concentration of base (or acid) is slowly added to a solution of acid (or base. ).. The . analyte. - is the solutions being evaluated and is of unknown concentration.. Amino acids are weak . polyprotic. acids . . Neutral amino acids are (. gly. , ala, threonine ) are treated as diprotic acids .. acidic amino acids (. glu. , asp,) are treated as . triprotic. acids .. pUC 1 18 Product Information Sheet # V333 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC 1 18 has four essential parts: . a method for . generating the DNA fragment for cloning; . A reaction . that inserts that fragment into the . chosen cloning vector. . a . means for introducing . that recombinant . .. Acid-Base Titration. .. المحاضرة الثانية للجزء العملي. . . Acid-Base Titration. . Analytical chemistry. : is the analysis of material samples to understanding of their chemical composition and structure. It has a wide range of monitoring pollution in the environment, development of new materials, and drug manufacture.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. .