PPT-DNA cloning with single stranded DNA vectors

M13 f1 and fd are filamentous coliphages containing a circular singlestranded DNA molecule These coliphages have been developed as cloning vectors for they have a number . @kBT df@kBT#G, Abbreviations:ds,double-stranded;ss,single-stranded;AFM,atomicforcemicroscopy.*Towhomreprintrequestsshouldbeaddressed.E-mail:cocco@franklin.phy.uic.edu.Permanentaddress:CentreNationalde Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. Copyright 2016 by the Rector and Visitors of the University of VirginiaRNA nucleotides or building blocks are structurally very similar to DNA and go by similar names The only exception is that RNA ha A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Jasmin . Sutkovic. Principles . of cloning, vectors and cloning strategies. by . Jasmin SUTJOVIC. Principles of cloning, vectors and cloning strategies. DNA CLONING. DNA cloning is a technique for reproducing DNA fragments. . Mammalian Cells. Gang Zhang, Ph. D. Research Technician II. Centre for Research in Neurodegenerative Diseases. Department of Medicine. University of Toronto, Canada. This talk based on the following publications:. pUC 1 18 Product Information Sheet # V333 02 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E - Mail: info@mobitec.com www.mobitec.com Revised January 2014 1 Product p UC 1 18 DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . A crucial property of the double helix is the capacity is to separate the two strands without disrupting the covalent bonds that form the . poly nucleotides . and at the very rapid rates needed to sustain genetic functions.. . Nucleases. . Methylases. and . Demethylases. . Phosphatases. and . Kinases. Polymerases. . Ligases. 2. Topology Modifiers. . Topoisomerases. . DNA Modifying Enzymes. Nucleases: . A . nuclease. A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . Halary S, Duraisamy R, Fancello L, Monteil-Bouchard S, Jardot P, Biagini P, et al. Novel Single-Stranded DNA Circular Viruses in Pericardial Fluid of Patient with Recurrent Pericarditis. Emerg Infect Dis. 2016;22(10):1839-1841. https://doi.org/10.3201/eid2210.160052.