PDF-Gene cloning

Principle Requirements steps and applications Dr Sudha Kumari Department of Veterinary Microbiology Bihar Veterinary College Patna Bihar Animal Sciences Un. (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . Chromosome. —. A . string like. , gene-containing molecule in the nucleus of a . cell. Gene. —. The fundamental unit of biological . inheritance. Genome. —An organism’s entire complement of DNA. (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . Son. Genetic Engineering. It is the direct manipulation of an organism’s genome using modern DNA technology … also called genetic modification. A genome is the entirety of an organism’s hereditary information. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. Gene cloning and other techniques, collectively termed DNA technology, can be used to manipulate and analyze DNA and to produce useful new products and organisms.. In genetic engineering, bacterial restriction enzymes are used to cut DNA molecules within short, specific nucleotide sequences (restriction sites), yielding a set of double-stranded DNA fragments with single stranded sticky ends.. AND ITS ETHICAL ISSUES Early Successes - Human Cloning  2001 – First cloned human embryos (only to six cell stage) created by Advanced Cell Technology (USA)  2004* – Claim of first h DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. . is the production of identical copies of organisms, cells or DNA. . A . clone. is a genetically identical organism or a group of genetically identical cells derived from a single parent.. Cloning occurs in nature as part of growth and reproduction. This natural process can be manipulated to produce clones of organisms artificially.. Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .