Chapter 5 Investigating DNA Major Topics 51 DNA Isolation Sequencing and Synthesis 52 Bioinformatics 53 Cloning and Recombinant Expression 54 Microarrays 55 In Situ Hybridization Foundations of Biochemistry ID: 912601
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Slide1
Foundations of Biochemistry
Chapter 5: Investigating DNA
Slide2Major Topics
5.1 DNA Isolation, Sequencing, and Synthesis
5.2 Bioinformatics
5.3 Cloning and Recombinant Expression
5.4 Microarrays
5.5 In Situ Hybridization
Slide3Foundations of Biochemistry
Chapter 5.2 Bioinformatics
Slide4Genome Sequencing Projects
DNA
—Photo by Flickr user MIKI Yoshihito
Slide5Bioinformatics
Collect statistics from biological data
Build a computational model
Solve a computational modeling problem
Test and evaluate a computational algorithm
Slide6Bioinformatics
Types of biological information and databases
Sequence analysis and molecular modeling
Genomic analysis
Systems biology
Slide7Figure from
Xiong
, X., et al. (2017) Front. Microbiol 8:2500
Genomics
Comparative Genomics
Proteomics
Slide8Applications of Genomics
Figure from
Ayday
, E. 2013
Slide9Foundations of Biochemistry
Chapter 5.3 Cloning and Recombinant Expression
Slide10Gel Electrophoresis
Slide11Cloning Vectors
Slide12Cloning Vectors
Origin of Replication (
ori
)
Multiple Cloning Site (MCS) or
polylinker
Selectible
Markers
Slide13Restriction Enzymes
Slide14Cloning into the Polylinker Site
Figure from
: Kahn Academy
Slide15Elements for Protein Expression
Promoter for RNA Polymerase binding
RBS – Ribosomal Binding Site for protein translation
Slide16Reporter Genes
Slide17Summary of DNA Cloning
Slide18Summary of DNA Cloning
Slide19Summary of DNA Cloning
Slide20Summary of DNA Cloning
Slide21Types of Vectors
Plasmids
Bacteriophages
Cosmids
Bacterial Artificial Chromosomes (BACs)
Yeast Artificial Chromosomes (YACs)
Human
Articicial
Chromosomes (HACs)
Slide22Cloning Vectors
Slide23Bacteriophages
Slide24Cosmids
Slide25BACs, YACs, and HACs
Slide26Reproductive Cloning
Slide27Foundations of Biochemistry
Chapter 5: Investigating DNA
Slide28Major Topics
5.1 DNA Isolation, Sequencing, and Synthesis
5.2 Bioinformatics
5.3 Cloning and Recombinant Expression
5.4 Microarrays
5.5 In Situ Hybridization
Slide29Genetic Engineering
CRISPR Technology
Slide30CRISPR
Slide31CRISPR-CAS Editing System
Figure from:
Mariuswalter
Slide32CRISPR/Cas
Figure from:
Mariuswalter
Slide33CRISPR/Cas Expansion
Figure from:
Mariuswalter
Slide342020 Nobel Prize in Chemistry
Emmanuelle Charpentier and Jennifer A. Doudna
Slide35Foundations of Biochemistry
Chapter 5.4 Microarrays
Slide36DNA Microarrays
Slide37DNA Microarrays
Slide38Foundations of Biochemistry
Chapter 5.5 In Situ Hybridization
Slide39In Situ Hybridization