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Foundations of Biochemistry Foundations of Biochemistry

Foundations of Biochemistry - PowerPoint Presentation

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Foundations of Biochemistry - PPT Presentation

Chapter 5 Investigating DNA Major Topics 51 DNA Isolation  Sequencing and Synthesis 52 Bioinformatics 53 Cloning and Recombinant Expression 54 Microarrays 55 In Situ Hybridization Foundations of Biochemistry ID: 912601

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Presentation Transcript

Slide1

Foundations of Biochemistry

Chapter 5: Investigating DNA

Slide2

Major Topics

5.1 DNA Isolation,  Sequencing, and Synthesis

5.2 Bioinformatics

5.3 Cloning and Recombinant Expression

5.4 Microarrays

5.5 In Situ Hybridization

Slide3

Foundations of Biochemistry

Chapter 5.2 Bioinformatics

Slide4

Genome Sequencing Projects

DNA

—Photo by Flickr user MIKI Yoshihito

Slide5

Bioinformatics

Collect statistics from biological data

Build a computational model

Solve a computational modeling problem

Test and evaluate a computational algorithm

Slide6

Bioinformatics

Types of biological information and databases

Sequence analysis and molecular modeling

Genomic analysis

Systems biology

Slide7

Figure from

Xiong

, X., et al. (2017) Front. Microbiol 8:2500

Genomics

Comparative Genomics

Proteomics

Slide8

Applications of Genomics

Figure from

Ayday

, E. 2013

Slide9

Foundations of Biochemistry

Chapter 5.3 Cloning and Recombinant Expression

Slide10

Gel Electrophoresis

Slide11

Cloning Vectors

Slide12

Cloning Vectors

Origin of Replication (

ori

)

Multiple Cloning Site (MCS) or

polylinker

Selectible

Markers

Slide13

Restriction Enzymes

Slide14

Cloning into the Polylinker Site

Figure from

: Kahn Academy

Slide15

Elements for Protein Expression

Promoter for RNA Polymerase binding

RBS – Ribosomal Binding Site for protein translation

Slide16

Reporter Genes

Slide17

Summary of DNA Cloning

Slide18

Summary of DNA Cloning

Slide19

Summary of DNA Cloning

Slide20

Summary of DNA Cloning

Slide21

Types of Vectors

Plasmids

Bacteriophages

Cosmids

Bacterial Artificial Chromosomes (BACs)

Yeast Artificial Chromosomes (YACs)

Human

Articicial

Chromosomes (HACs)

Slide22

Cloning Vectors

Slide23

Bacteriophages

Slide24

Cosmids

Slide25

BACs, YACs, and HACs

Slide26

Reproductive Cloning

Slide27

Foundations of Biochemistry

Chapter 5: Investigating DNA

Slide28

Major Topics

5.1 DNA Isolation,  Sequencing, and Synthesis

5.2 Bioinformatics

5.3 Cloning and Recombinant Expression

5.4 Microarrays

5.5 In Situ Hybridization

Slide29

Genetic Engineering

CRISPR Technology

Slide30

CRISPR

Slide31

CRISPR-CAS Editing System

Figure from:

Mariuswalter

Slide32

CRISPR/Cas

Figure from:

Mariuswalter

Slide33

CRISPR/Cas Expansion

Figure from:

Mariuswalter

Slide34

2020 Nobel Prize in Chemistry

Emmanuelle Charpentier and Jennifer A. Doudna

Slide35

Foundations of Biochemistry

Chapter 5.4 Microarrays

Slide36

DNA Microarrays

Slide37

DNA Microarrays

Slide38

Foundations of Biochemistry

Chapter 5.5 In Situ Hybridization

Slide39

In Situ Hybridization