PPT-RESTRICTION ENZYMES AND VECTORS

GENETIC ENGINEERING Genetic engineering is the manipulation of genetic materials which can be introduced in the host organisms and thus change the phenotype of the host organism. Stephen. . Halford. DNA-Proteins Interactions Unit,. Department of Biochemistry,. Why study the . enzymology. of Type II restriction enzymes?. . Enzyme specificity. . c.f. . aminoacyl. . tRNA. . Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Restriction enzymes. Polymerase enzyme. Strand-displacing polymerases. Helicase enzymes. Discovery and Obtainability:. Most enzymes are proteins discovered in cells.. But . DNAzymes. were . descovered. are. . working. ?. Restriction. Enzymes. Natural . occurrence. . of. . restriction. . enzymes. . is. in . bacteria. . for. . protecting. . them. . against. a . virus. . attack. . by. . Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Today’s Experiment. Restriction Enzymes allow researchers precisely cut DNA. : An enzymes that breaks down nucleic acid molecule by breaking the phosphodiester bonds at end 3. / . or 5. /.. Endonucleases. : . An Enzyme that hydrolyzes internal phosphodiester bonds in a polynucleotide chain or nucleic acid molecules.. Restriction Modification System. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. 1. https://. socratic.org. /questions/why-are-restriction-enzymes-important-for-recombinant-dna-technology. Restriction enzymes sometimes called molecular scissors . PART 1: Background. What do restriction enzymes do . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Types of R.E.:. by. Prof. . Meena. . Nagawanshi. Professor . Department of . Zoology. Deogiri . College, Aurangabad. Enzymes. Enzymes are proteins. . biological catalysts .  help drive biochemical reactions. Enzyme names end with an .