PPT-Chapter 4: Restriction endonucleases

1 https socraticorg questionswhyarerestrictionenzymesimportantforrecombinantdnatechnology Restriction enzymes sometimes called molecular scissors PART 1 Background What do restriction enzymes do . GENETIC. . ENGINEERING. Genetic engineering is the manipulation of genetic materials which can be introduced in the host organisms and thus change the phenotype of the host organism.. Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . Site-specific restriction endonucleases are used to identify DNA molecules. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . Protocol 10.1 through 10.3. Objective. : To cut phage genome into multiple fragments based on DNA sequence. General Introduction on Restriction Enzymes. Are also known as restriction endonucleases. Are naturally occurring enzymes used by bacteria for defensive purposes against extraneous DNA molecules. factors . increase. in response . to. . viral. rebound . after. . analytical. treatment . interruption. . De Scheerder Marie-Angélique, Van Hecke . Clarissa. , De . Langhe. Nele, Sips Magdalena, . Tab C- abridged sequence. Starts on page C-1. Student. Introduction. Reading: . Plasmids and Restriction Enzymes. Activity: . Clone that gene. Laboratory 2a: . Preparing to verify the . rfp. gene: digesting the . Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Enzymes“. „. Sequence. . specific. . recognition. . and. . engineering. “. Alfred Pingoud. CSHL. Oct. . 19-21 2013. roberts@neb.com. . Sa 03.08.2013 . Outline of . talks. Alfred . Pingoud (25 . Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Today’s Experiment. Restriction Enzymes allow researchers precisely cut DNA. the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603. by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Types of R.E.:. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Mechanical shearing.