PPT-History of Restriction Enzymes

Meeting Summary Stu Linn University of California Berkeley Some Items Noted for Restriction and Modification Studies   There were a number of small meetings devoted especially to restriction and modification . Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. An Introduction to Restriction Enzymes & Gel Electrophoresis. Objectives. Understand the use of restriction enzymes as biotechnology tools. Become familiar with principles and techniques of . agarose. Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. Dr. Kevin Ahern. Protease Mechanisms. Aspartic Acid. Aspartic Acid. Histidine. Cysteine. Electron rich. Metal. Other Protease Families. Cysteine. Aspartyl. Metallo-. Papain. Cathepsin D. Carboxypeptidase A. Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . Vocabulary. Karyotype. Autosome. Sex chromosome. Nondisjunction. Monosomy. Trisomy. Pedigree. Carrier. Restriction enzyme. Restriction site. Restriction fragment. Sticky ends. Recombination. Gel electrophoresis. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Enzymes“. „. Sequence. . specific. . recognition. . and. . engineering. “. Alfred Pingoud. CSHL. Oct. . 19-21 2013. roberts@neb.com. . Sa 03.08.2013 . Outline of . talks. Alfred . Pingoud (25 . Restriction enzymes. Polymerase enzyme. Strand-displacing polymerases. Helicase enzymes. Discovery and Obtainability:. Most enzymes are proteins discovered in cells.. But . DNAzymes. were . descovered. are. . working. ?. Restriction. Enzymes. Natural . occurrence. . of. . restriction. . enzymes. . is. in . bacteria. . for. . protecting. . them. . against. a . virus. . attack. . by. . Restriction Modification System. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. 1. https://. socratic.org. /questions/why-are-restriction-enzymes-important-for-recombinant-dna-technology. Restriction enzymes sometimes called molecular scissors . PART 1: Background. What do restriction enzymes do . Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Mechanical shearing. by. Prof. . Meena. . Nagawanshi. Professor . Department of . Zoology. Deogiri . College, Aurangabad. Enzymes. Enzymes are proteins. . biological catalysts .  help drive biochemical reactions. Enzyme names end with an .