PPT-Enzymes Chapter 3 Enzymes

Part 1 Chapter 3 Learning outcomes You should be able to explain how enzymes work describe and explain the factors that affect enzyme activity use V max and K m to compare the affinity of different enzymes for their substrates. And 57375en 57375ere Were None meets the standard for Range of Reading and Level of Text Complexity for grade 8 Its structure pacing and universal appeal make it an appropriate reading choice for reluctant readers 57375e book also o57373ers students Definition of an enzyme. Enzyme. is . protein . . catalyst. (i.e. . increase the rate of reactions. ). . NOT. changed during the reaction. ch. 12. The protein catalyst. Targets:. Describe the relationship between substrate and an active site in enzymatic reactions. Identify the role of coenzymes and enzymatic reactions. List factors that affect enzymatic activity. Reactions. Most reactions take place in a number of steps which need to be carefully controlled if the cell is to function properly. Enzymes are the most important controllers of cellular reactions. Catalysts. Enzymes as Catalysts. Enzymes are used to speed up chemical (metabolic) reactions e.g. respiration or photosynthesis- so why use enzymes in industry?. They are specific. can catalyse reactions between specific chemicals, even in a large mixture . Essential idea: Enzymes control the metabolism of the cell.. Above is just a small part of the IUBMB-. Sigma-Nicholson Metabolic Pathways Chart . aims to show all the metabolic pathways found in eukaryote cells. The chart in it's entirety shows how complex the chemicals reactions needed to support life in a single cell unit. This was mentioned earlier in 2.1, but in addition to the complexity now add to that . Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . 6.4 Cloning & Biotechnology. Learning Objectives. Understand the methods used for immobilising enzymes.. Understand the advantages of using immobilised enzymes.. Enzymes as Catalysts. Enzymes are used to speed up chemical (metabolic) reactions e.g. respiration or photosynthesis- so why use enzymes in industry?. Are specific for . what they . catalyze . and do not permanently change during reactions. End in the word -. ase. Function of Enzymes. Enzymes work by lowering the activation energy (the amount of energy needed to start a reaction). . 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. protein. !. Enzymes are . Biological . catalysts . that . increase. . the rate of metabolic reactions.. Since enzymes speed up chemical reaction rates, what are they called?. Catalyst- . A substance . Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . : An enzymes that breaks down nucleic acid molecule by breaking the phosphodiester bonds at end 3. / . or 5. /.. Endonucleases. : . An Enzyme that hydrolyzes internal phosphodiester bonds in a polynucleotide chain or nucleic acid molecules.. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:.