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Figure 3 Figure 3. Restriction fragment length polymorphism (RFLP) analysis of Figure 3 Figure 3. Restriction fragment length polymorphism (RFLP) analysis of

Figure 3 Figure 3. Restriction fragment length polymorphism (RFLP) analysis of - PowerPoint Presentation

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Figure 3 Figure 3. Restriction fragment length polymorphism (RFLP) analysis of - PPT Presentation

Choi Y Jang W Kim J Ryu J Lee S Park K et al Spotted Fever Group and Typhus Group Rickettsioses in Humans South Korea Emerg Infect Dis 2005112237244 httpsdoiorg103201eid1102040603 ID: 1014901

restriction dna fragments nested dna restriction nested fragments group reactions set primer amplified size digestion

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1. Figure 3Figure 3. Restriction fragment length polymorphism (RFLP) analysis of H2-products amplified with multiplex-nested primer set from seropositive sera. Ethidium bromide–stained polyacrylamide gels of AluI restriction endonuclease digestion of ≈230-bp rickettsial DNA amplified by using the nested primer H set WJ77/80 in the primary reactions and WJ79/83/78 in the nested reactions. Lanes: M, size marker DNA (25-bp DNA ladder); 1, H3-2; 2, H7-2; 3, H8-2; 4, H13-2; 5, H14-2; 6, H15-2; 7, H18-2; 8, H19; P, R. prowazekii; T, R. typhi. P and T; predicted fragments after digestion. The number on the left indicates the molecular size (in base pairs) of restriction fragments.Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603