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PPT-Figure Figure. Multilocus polymerase chain reaction–restriction fragment length

PPT-Figure Figure. Multilocus polymerase chain reaction–restriction fragment length

Author : mackenzie | Published Date : 2024-01-13

Cama V Gilman RH Vivar A Ticona E Ortega Y Bern C et al Mixed Cryptosporidium Infections and HIV Emerg Infect Dis 200612610251028 httpsdoiorg103201eid1206060015

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Figure Figure. Multilocus polymerase chain reaction–restriction fragment length: Transcript

Cama V Gilman RH Vivar A Ticona E Ortega Y Bern C et al Mixed Cryptosporidium Infections and HIV Emerg Infect Dis 200612610251028 httpsdoiorg103201eid1206060015. Nahla . Bakhamis. Multiple copies of specific DNA . sequences;. . ‘Molecular Photocopying’ . Polymerase Chain Reaction. 1983;. In . vitro. enzymatic amplification of specific DNA sequences from . Kochar DK, Saxena V, Singh N, Kochar SK, Kumar SV, Das A. Plasmodium vivax Malaria. Emerg Infect Dis. 2005;11(1):132-134. https://doi.org/10.3201/eid1101.040519. Murase Y, Maeda S, Yamada H, Ohkado A, Chikamatsu K, Mizuno K, et al. Clonal Expansion of Multidrug-Resistant and Extensively Drug-Resistant Tuberculosis, Japan. Emerg Infect Dis. 2010;16(6):948-954. https://doi.org/10.3201/eid1606.091844. Morré SA, Spaargaren J, Fennema J, de Vries H, Coutinho RA, Peña A. Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum. Emerg Infect Dis. 2005;11(8):1311-1312. https://doi.org/10.3201/eid1108.050535. Alberti A, Addis M, Sparagano O, Zobba R, Chessa B, Cubeddu T, et al. Anaplasma phagocytophilum, Sardinia, Italy. Emerg Infect Dis. 2005;11(8):1322-1324. https://doi.org/10.3201/eid1108.050085. Jhung MA, Thompson AD, Killgore GE, Zukowski WE, Songer G, Warny M, et al. Toxinotype V Clostridium difficile in Humans and Food Animals. Emerg Infect Dis. 2008;14(7):1039-1045. https://doi.org/10.3201/eid1407.071641. Figure 6.01. Polymerase Chain Reaction (PCR). During PCR, two primers anneal to complementary sequences at either end of a target sequence on a piece of denatured sample DNA. . DNA polymerase. synthesizes a complementary strand of DNA from the primers, resulting in two new strands of DNA. In further cycles, the newly made DNA molecules are denatured, primers are annealed, and DNA polymerase copies the target regions, resulting in multiple copies of the original target sequence.. Jain SK, Persaud D, Perl TM, Pass MA, Murphy KM, Pisciotta JM, et al. Nosocomial Malaria and Saline Flush. Emerg Infect Dis. 2005;11(7):1097-1099. https://doi.org/10.3201/eid1107.050092. Currie BJ, Haslem A, Pearson T, Hornstra H, Leadem B, Mayo M, et al. Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis. Emerg Infect Dis. 2009;15(2):169-174. https://doi.org/10.3201/eid1502.081036. Figure 8.01. Sequencing—Fragments of All Possible Lengths. During chain termination or . Sanger sequencing. , the target DNA fragments are copied millions of times, but each copy ends at a different nucleotide position. These subsets of fragments end with a fluorescently-labeled nucleotide that reveal the identity of the final base. The final sequencing data are a series of fluorescent peaks that correspond to the original template . Ranque S, Faugère B, Pozio E, La Rosa G, Tamburrini A, Pellissier J, et al. Trichinella pseudospiralis Outbreak in France. Emerg Infect Dis. 2000;6(5):543-547. https://doi.org/10.3201/eid0605.000517. Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603. Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ. Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay. Emerg Infect Dis. 2001;7(4):739-741. https://doi.org/10.3201/eid0704.017425. Kariuki S, Gilks C, Revathi G, Hart CA. Genotypic Analysis of Multidrug-Resistant Salmonella enterica Serovar Typhi, Kenya. Emerg Infect Dis. 2000;6(6):649-651. https://doi.org/10.3201/eid0606.000616.

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