PPT-Biotechnology Basics: Restriction Digests using Kit 225

Danielle R Snowflack PhD wwwedvotekcom EDVOTEK The Biotechnology Education Company Celebrating OVER 30 years of science education Todays Experiment Restriction Enzymes allow researchers precisely cut DNA. Digests group cases by topic and provide short summaries on what the cited cases say about t he given subject Digest sets cover cases from a specific jurisdiction or general subject area Topical digests such as the Bankruptcy and Real Estate digests CSCI 5857: Encoding and Encryption. Outline. Information integrity issues . Message digests . Hash functions. Insuring information integrity. Attacks on message digests. Preimage. attacks. Collision attacks. BY: Selena & Nicole. Foundations of biotechnology . Biotechnology can be defined as the manipulation of living organisms or their components to provide products or to serve useful purpose. As result the DNA of plants, animals and other organisms can be manipulated in the laboratory .. Checking off: . Notes . on . Ch 20.1, 20.2. With your group, discuss what you know about these:. Methylation and Acetylation. Genetic Engineering/Biotechnology. Restriction enzymes. Gel Electrophoresis. CSCI 5857: Encoding and Encryption. Outline. Attacks on message digests. Preimage. attacks. Collision attacks. Properties of a good hash function. Mathematical background. Pigeonhole principle. Birthday . Biotechnology. . In 2004, this child became known as Baby 81 when disaster struck his small village in Southeast Asia. After a devastating tsunami hit, he was rescued and months later reunited him with his biological parents.. Biotechnology: the branch of . molecular biology . that studies the use of . microorganisms. to perform specific industrial processes; "biotechnology produced . genetically altered . bacteria that solved the problem"  . Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . Explorer™ . DNA Fingerprinting Kit. Crime Scene. Have fun setting up your own crime scene. Be as elaborate or as simple as you wish.. Dye Electrophoresis . Could you eliminate any suspects using dye electrophoresis?. the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Biotechnology. Using organisms and biological processes for human benefit. Very old. Fermentation to produce alcohol and bread. New techniques allow manipulation at the molecular level. Some biotechnology controversial . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . learning objectives. Understand what is meant by genetic engineering.. Understand the steps involved.. Understand what restriction enzymes do.. Understand why sticky ends are important.. success criteria.