PPT-HIV-1 specific restriction

factors increase in response to viral rebound after analytical treatment interruption De Scheerder MarieAngélique Van Hecke Clarissa De Langhe Nele Sips Magdalena . Insertion-deletion length polymorphism – INDEL. Single nucleotide polymorphism – SNP. Simple sequence repeat length polymorphism – mini- and micro-satellites . Labeled 3’ TGGCTAGCT 5’. Richard J. Roberts. New England Biolabs. CSHL - . 2013. AHA!. Danna, K., Nathans, D. (1971) Specific cleavage of simian virus 40 DNA by restriction endonuclease of . Hemophilus. influenza. . Proc. Natl. Acad. Sci. USA 68: 2913-2917. . Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . Site-specific restriction endonucleases are used to identify DNA molecules. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Enzymes“. „. Sequence. . specific. . recognition. . and. . engineering. “. Alfred Pingoud. CSHL. Oct. . 19-21 2013. roberts@neb.com. . Sa 03.08.2013 . Outline of . talks. Alfred . Pingoud (25 . are. . working. ?. Restriction. Enzymes. Natural . occurrence. . of. . restriction. . enzymes. . is. in . bacteria. . for. . protecting. . them. . against. a . virus. . attack. . by. . Restriction enzymes . Restriction enzymes . (or restriction endonucleases), are bacterial enzymes that cleave both strands of DNA at or near specific recognition nucleotide sequences (4 -8 bp ) known as restriction sites. . the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Restriction Modification System. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. 1. https://. socratic.org. /questions/why-are-restriction-enzymes-important-for-recombinant-dna-technology. Restriction enzymes sometimes called molecular scissors . PART 1: Background. What do restriction enzymes do . Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Mechanical shearing.