PPT-Restriction Enzyme digestion of DNA - Exercise 8

Objectives Understand how Restriction Enzymes digest DNA Know how to construct a pAMP plasmid or gel Given the size of fragments gel know how to construct a restriction map Given a restriction map know how to construct a gel. Richard J. Roberts. New England Biolabs. CSHL - . 2013. AHA!. Danna, K., Nathans, D. (1971) Specific cleavage of simian virus 40 DNA by restriction endonuclease of . Hemophilus. influenza. . Proc. Natl. Acad. Sci. USA 68: 2913-2917. . Restriction Enzymes. General Genetics. Objectives:. Introduce the students to digest genomic DNA by restriction . endonucleases. .. Observe the results of digestion on . agarose. gel electrophoresis.. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Using Restriction Enzymes. Learning targets for Restriction . Digestion and Analysis of Lambda DNA . lab?. I will understand . the use of restriction enzymes as biotechnology . tools. I will become . The process in which food is . broken down . into smaller . molecules. that can be . absorbed. into the body and are . usable by cells. .. Five Main Digestive Processes. Ingestion. – Eat; . . Intaking. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Explorer™ . DNA Fingerprinting Kit. Crime Scene. Have fun setting up your own crime scene. Be as elaborate or as simple as you wish.. Dye Electrophoresis . Could you eliminate any suspects using dye electrophoresis?. Primer Design Molecular cloning requires manipulation of nucleic acid, including copying (synthesize), cutting (digest) and pasting (ligate). In bacteria, it is usually done in plasmid form. To inse the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Restriction Modification System. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. 1. https://. socratic.org. /questions/why-are-restriction-enzymes-important-for-recombinant-dna-technology. Restriction enzymes sometimes called molecular scissors . PART 1: Background. What do restriction enzymes do . Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603. Cutting DNA molecules. . Before 1970s, there was no method of cleaving DNA at the specific points.. All the available methods for fragmenting DNA were non-specific.. Mechanical shearing.