PPT-Recombinant DNA Technology

PLASMID VECTORS Cloning into a Plasmid Bacteria are useful hosts They are easily grown They are cheap to grow They grow fast They are easily manipulated in the laboratory DNA can be inserted transformation. Dr. Brian Rymond (Instructor). &. Christen . Wanstrath. (TA). Make and break DNA (and RNA) in a variety of ways and test the consequences in the host organism (. E. coli, S. cerevisiae, C. elegans. DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock Definition. Steps . Applications. INTRODUCTION. Recombinant DNA.  (. rDNA. ): .  . DNA.  molecules formed by laboratory methods of . genetic recombination.  (such as . molecular cloning. ) to bring together genetic material from multiple sources, creating . BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Construction & Cloning of Recombinant DNA. From Campbell Biology, . Chp. . 20. Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s TECHNOLOGY 1 Objectives • Recombinant DNA • Probes • Restriction map • Gene cloning • Gene library • Cloning vectors • RFLP • DNA Fingerprinting • DNA foot printing • Genomic impri R ecombinant VACCINE  Ability to elicit the appropriate immune response for a particular pathogen.  Long term protection  Safety  Stability  inexpensiveness RECOMBINANT VACCINE  A r 683 Human Erythropoietin (hEPO) is the main hormone involved in the differentiation, proliferation and maintaining of physiologic levels of erythroid stem cells, it was also the rst hematopoietic great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.