PPT-3 Basic Principles of Recombinant DNA Technology

Brief Chapter Outline   I Cause for Concern Recombinant DNA Technology Promise and Controversy Cutting and Joining DNA DNA Cloning V Cloning Vectors A Bacterial Vectors. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . . Hameed. Abdullah. (. or . rDNA. ) is made by combining DNA from two or more sources. In practice, the process often involves combining the DNA of different organisms. . The . process depends on the ability of cut, and re-join, DNA molecules at points identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using . Genetic engineering involves inserting foreign genes or modifying the activity of existing genes. . This technology exploits the process of gene cloning which involves making many identical copies of a gene. . Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. Concept and basic steps in recombinant DNA technology and gene cloning. -2-. Recombinant DNA technology is playing a vital role in improving health conditions by . developing new vaccines and pharmaceuticals. TECHNOLOGY 1 Objectives • Recombinant DNA • Probes • Restriction map • Gene cloning • Gene library • Cloning vectors • RFLP • DNA Fingerprinting • DNA foot printing • Genomic impri R ecombinant VACCINE  Ability to elicit the appropriate immune response for a particular pathogen.  Long term protection  Safety  Stability  inexpensiveness RECOMBINANT VACCINE  A r 683 Human Erythropoietin (hEPO) is the main hormone involved in the differentiation, proliferation and maintaining of physiologic levels of erythroid stem cells, it was also the rst hematopoietic great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . (. Cellular and molecular pharmacology. ) . Chhatrapati Sahu Ji Maharaj University, Kanpur. 1. CSJM UNIVERSITY, KANPUR. Contents. Recombinant DNA Technology. Introduction. Goals and Objectives. R-DNA technology procedure. Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms.