PPT-Genetic Engineering (Recombinant DNA technology)

Genetic engineering involves inserting foreign genes or modifying the activity of existing genes This technology exploits the process of gene cloning which involves making many identical copies of a gene . Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Mutation. Permanent change in the genetic material of an organism. All mutations in gametes are inheritable because they are copied during DNA replication. Somatic cell mutations occur in body cells. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. What we want to learn!. Summarize how transgenic organisms are engineered to benefit society.. Explain how transgenic organisms are used in agriculture, industry, and medicine.. Selective Breeding. For thousands of years, humans have selected plants and animals with certain qualities or desired traits.. Dr. Brian Rymond (Instructor). &. Christen . Wanstrath. (TA). Make and break DNA (and RNA) in a variety of ways and test the consequences in the host organism (. E. coli, S. cerevisiae, C. elegans. Introduction and Application in Genetic Engineering. Genetic engineering. , . the artificial manipulation, modification, and recombination of DNA or other nucleic acid molecules in order to modify an organism or population of organisms. . Stephen Taylor. http://sciencevideos.wordpress.com. 4.4 Genetic Engineering & Biotechnology. 1. Polymerase Chain Reaction. Used to . amplify small samples of DNA. In order to use them for DNA profiling, recombination, species identification or other research. . Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s (. Cellular and molecular pharmacology. ) . Chhatrapati Sahu Ji Maharaj University, Kanpur. 1. CSJM UNIVERSITY, KANPUR. Contents. Recombinant DNA Technology. Introduction. Goals and Objectives. R-DNA technology procedure. Technology. . and. . Biotechnology. II. . FE314. -. . Biotechnology. Spring . 201. 6. Some. . applications. of . recombiant. DNA . technology. in . Biotechnology. *. Diabates-Insulin. . production. ?. G. enetic . engineering. , the artificial manipulation, modification, and recombination of DNA or other nucleic acid molecules in order to modify an organism or population of organisms. . This . may mean changing one base . Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms.