PDF-Recombinant DNA

Technology AQC 321 Dr Mamta Singh Assistant Professor COF BASU Kishanganj Recombinant DNA Technology Definition It is a technology of joining together of DNA molecules from two different s. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Will Herrick. Peyton Group Meeting. March 20, 2013. What Are Adenoviruses?. Linear . dsDNA. virus. 80-100 nm. Nonenveloped. Icosahedral structure:. 1: penton . capsomeres. 2: . hexon. . capsomeres. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock R. r. Source: plant-genetics.kais.kyoto-u.ac.jp. ATGGGCACTGCCACT. A chromosome is a single DNA molecule complexed with histone proteins. DNA is highly compacted: DNA + histone proteins = . chromatin. . Hameed. Abdullah. (. or . rDNA. ) is made by combining DNA from two or more sources. In practice, the process often involves combining the DNA of different organisms. . The . process depends on the ability of cut, and re-join, DNA molecules at points identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using . Definition. Steps . Applications. INTRODUCTION. Recombinant DNA.  (. rDNA. ): .  . DNA.  molecules formed by laboratory methods of . genetic recombination.  (such as . molecular cloning. ) to bring together genetic material from multiple sources, creating . www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Construction & Cloning of Recombinant DNA. From Campbell Biology, . Chp. . 20. R ecombinant VACCINE  Ability to elicit the appropriate immune response for a particular pathogen.  Long term protection  Safety  Stability  inexpensiveness RECOMBINANT VACCINE  A r 683 Human Erythropoietin (hEPO) is the main hormone involved in the differentiation, proliferation and maintaining of physiologic levels of erythroid stem cells, it was also the rst hematopoietic great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . (. Cellular and molecular pharmacology. ) . Chhatrapati Sahu Ji Maharaj University, Kanpur. 1. CSJM UNIVERSITY, KANPUR. Contents. Recombinant DNA Technology. Introduction. Goals and Objectives. R-DNA technology procedure. Technology. . and. . Biotechnology. II. . FE314. -. . Biotechnology. Spring . 201. 6. Some. . applications. of . recombiant. DNA . technology. in . Biotechnology. *. Diabates-Insulin. . production.