PPT-genetic engineering module 2 – biotechnology & gene technologies

learning objectives Understand what is meant by genetic engineering Understand the steps involved Understand what restriction enzymes do Understand why sticky ends are important success criteria. Essential idea: Biologists have developed techniques for artificial manipulation of DNA, cells and organisms.. There are a number of key techniques involved in the analysis of DNA and gene transfer. The image above shows nuclear transfer, the key step in cloning by somatic cell nuclear transfer. Dolly the sheep was the first success clone using this technique of direct cell manipulation. SCNT remains a key part of genetic engineering as it allows patient specific embryonic stem cells to be created, a key step in the application of gene therapies in a patient.. Biotechnology. . In 2004, this child became known as Baby 81 when disaster struck his small village in Southeast Asia. After a devastating tsunami hit, he was rescued and months later reunited him with his biological parents.. At the end of this lesson you should be able to . Define Genetic Engineering. Outline the process of genetic engineering involving some or all of the following: isolation, cutting, transformation, introduction of base sequence changes and expression. Genetic Engineering. 1. ) Inserting a new a gene. 2) Altering a gene. 3) Deleting or turning off a gene (gene knockout). Tools. Three important tools used for genetic engineering. PCR. : takes a single copy of the gene of interest and makes . Stephen Taylor. http://sciencevideos.wordpress.com. 4.4 Genetic Engineering & Biotechnology. 1. Polymerase Chain Reaction. Used to . amplify small samples of DNA. In order to use them for DNA profiling, recombination, species identification or other research. . of. Biotechnology. Dr. Habibur Rahman. Associate Professor. J. N. College, Boko. Introduction . to Biotechnology:. The root words of biotechnology are ancient Greek:• Bios: "life"• . Technikos. : “Skillfully made "tool"• Logos: "Study of," "word," "essence". Applying Genetics. Name some Dog breeds…. Terms:. Selective breeding. Domestication. Hybridization. Inbreeding. Purebreds . increased recessive traits. Is selective breeding a form of genetic engineering?. Biotechnology The term “ Biotechnology" was first coined in 1919 by Karl Ereky which means p roducts are produced from raw materials wi th the aid of living organisms. Biotechnology is NOT new. M ?. G. enetic . engineering. , the artificial manipulation, modification, and recombination of DNA or other nucleic acid molecules in order to modify an organism or population of organisms. . This . may mean changing one base . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Specification Reference. Learning Objective. Understand the principles of genetic engineering. . Understand the techniques used in genetic engineering.. Success Criteria. Describes the steps involved in isolating and then transferring a gene into a vector.. We have been manipulating DNA for generations!. Selective breeding. Allowing only those animals/plants with desired characteristics to produce the next . generation (foods, vaccines, . bidegeneration. Sciences. By. Swarnakshi. . Upadhyay. Assistant Professor. School of Pharmaceutical Sciences. DEFINITION. It is defined as the application of scientific and engineering principles to the processing of material by biological agents to provide good services.. Gene therapy . Genetic Engineering. Use the website to research the answers to the questions on your sheet.. Be ready to discuss the answers next lesson.. Using technology to modify organisms. Recombinant DNA technology can now be used to alter the genetic make-up of organisms much more quickly..